Anti-SDHD Rabbit Polyclonal Antibody for WB, ELISA - O14521

Core Product Specifications and Parameters

Biological Background: SDHD Function and Localization

• SDHD is a membrane-anchoring subunit of succinate dehydrogenase (complex II) in the mitochondrial inner membrane, essential for complex stability and function. Related references: PMID:10482792, PMID:9533030
• It plays a critical role in the electron transport chain by transferring electrons from succinate to ubiquinone (coenzyme Q). Related references: PMID:10482792, PMID:9533030
• SDHD also contributes to malate oxidation, converting malate to enol-oxaloacetate, a potent inhibitor of succinate dehydrogenase, which is then isomerized into keto-oxaloacetate. (By similarity)
• The protein is localized exclusively to the mitochondrial inner membrane, consistent with its predicted transmembrane domains.
• SDHD is known by multiple aliases, including PGL1, CII-4, and QPs3; mutations in the SDHD gene are linked to hereditary paraganglioma syndromes. (Keywords: Disease variant)
• As part of complex II, SDHD connects the tricarboxylic acid cycle to the mitochondrial respiratory chain, underscoring its metabolic importance. (Keywords: Tricarboxylic acid cycle)

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 50–150 of human SDHD. Peptide competition assays can be used to verify antibody specificity.
• For Western blotting, SDHD migrates at ~17 kDa; using a 12–15% polyacrylamide gel and appropriate molecular weight standards is recommended.
• This antibody has been validated for WB and ELISA; optimal working dilutions should be determined empirically for each application and sample type.
• Cross-reactivity with mouse and rat samples has been demonstrated, but validation in the specific experimental model is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibodies, antigens, and bulk reagents for mitochondrial and metabolism research. With this SDHD polyclonal antibody, researchers gain a reliable tool for complex II studies, supported by CamelBio’s end-to-end supply chain solutions. Our platform also accommodates rare-target raw material sourcing to accelerate diagnostic development from concept to clinic.