Anti-SDHC Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q99643

Core Product Specifications and Parameters

Biological Background: SDHC Function and Localization

• SDHC (also known as CYBL, QPs-1, SDH3) is a membrane-anchoring subunit of succinate dehydrogenase (Complex II) of the mitochondrial electron transport chain, responsible for transferring electrons from succinate to ubiquinone. Related references: PMID:9533030
• The SDH complex also catalyzes the oxidation of malate to the enol form of oxaloacetate, a potent inhibitor of succinate dehydrogenase activity, which is further isomerized into keto-oxaloacetate.
• Localized to the mitochondrial inner membrane, SDHC is an integral membrane protein containing transmembrane helices and heme cofactors for electron transfer.
• SDHC is a key component of the tricarboxylic acid (TCA) cycle and oxidative phosphorylation, linking succinate oxidation to the ubiquinone pool.
• The protein undergoes alternative splicing and contains transit peptide, heme-binding, and iron-sulfur cluster motifs critical for its function.
• Human SDHC has a calculated molecular weight of approximately 18.6 kDa, with the mature form often migrating at ~19 kDa on SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-100) of human SDHC; it is expected to detect both the precursor and mature forms.
• For Western blotting, consider using mitochondrial-enriched lysates from human, mouse, or rat tissues (e.g., heart, skeletal muscle) as positive controls; the expected band is ~19 kDa.
• For IHC-P and IF/ICC, validate the antibody in tissues or cell lines with high mitochondrial content; co-staining with a mitochondrial marker is recommended.
• Since the antibody is suitable for ELISA, it can be used for quantitative studies, but appropriate assay optimization and controls are needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—all supported by technical and consulting services from concept to clinic. For complex II and mitochondrial research, our SDHC Rabbit mAb provides a reliable reagent for diagnostic and laboratory applications. Contact us to explore custom sourcing or bulk supply options tailored to your development needs.