Anti-SDHC Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q99643

Core Product Specifications and Parameters

Biological Background: SDHC Function and Localization

• SDHC (succinate dehydrogenase cytochrome b560 subunit) is the membrane-anchoring subunit of succinate dehydrogenase (complex II) and is essential for mitochondrial electron transport.
• It transfers electrons from succinate to ubiquinone, linking the TCA cycle to the respiratory chain (PubMed:9533030). Related references: PMID:9533030
• SDH also oxidizes malate to enol-oxaloacetate, which isomerizes to keto-oxaloacetate, a reaction that may regulate SDH activity (By similarity).
• The SDHC gene encodes multiple isoforms through alternative splicing; it is also known as CYBL, QPS1, and SDH3.
• SDHC is an integral membrane protein localized to the mitochondrial inner membrane.
• It contains heme and iron cofactors and binds metal ions, which are required for electron transfer.
• Annotated with keywords including transit peptide, transmembrane helix, and tricarboxylic acid cycle, reflecting its mitochondrial import and function.

Experimental Guidance and Technical Tips

• The immunogen is a peptide from the N-terminal region (aa 1–100) of human SDHC; consider this when cross-referencing with sequence databases for mouse/rat homology.
• For WB, use mitochondrial-enriched fractions or whole cell lysates; a band around 19 kDa is expected.
• For IHC-P and IF/ICC, include appropriate antigen retrieval or permeabilization steps; validate in relevant tissues (e.g., heart, muscle, kidney).
• For ELISA, the antibody may be used as a capture or detection reagent; optimize coating and blocking conditions.
• As a polyclonal antibody, it may exhibit batch-to-batch variability; lot-specific validation is recommended.
• Cross-reactivity with human, mouse, and rat is stated; verify in your experimental system if targeting other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This SDHC polyclonal antibody supports studies of mitochondrial function, energy metabolism, and complex II-related diseases. For custom antibody development or rare-target sourcing, contact CamelBio for tailored solutions.