Anti-SDHB Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P21912

Core Product Specifications and Parameters

Biological Background: SDHB Function and Localization

• SDHB encodes the iron-sulfur (Ip) subunit of mitochondrial succinate dehydrogenase (Complex II), which is a key enzyme linking the Krebs cycle and oxidative phosphorylation.
• Core function: transfers electrons from succinate to ubiquinone (coenzyme Q) in the electron transport chain (PubMed:26925370, PubMed:27604842). Related references: PMID:26925370 PMID:27604842
• Also catalyzes the oxidation of malate to enol-oxaloacetate, a potent SDH inhibitor that isomerizes to keto-oxaloacetate (By similarity).
• Alternative names include Iron-sulfur subunit of complex II, SDH, SDH1; the gene is also known as SDH, CWS2, PGL4, among others.
• Subcellular localization: integral component of the mitochondrial inner membrane.
• The protein contains multiple iron-sulfur clusters (2Fe-2S, 3Fe-4S, 4Fe-4S) essential for its electron transfer function and is subject to acetylation.
• Associated with primary mitochondrial disease; mutations in SDHB are linked to hereditary paraganglioma and pheochromocytoma (Keywords: Disease variant, Primary mitochondrial disease).

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 29–280 of human SDHB, corresponding to the mature protein after mitochondrial import and processing; thus, the antibody is suitable for detecting endogenous SDHB across human, mouse, and rat samples.
• For Western blotting, an expected band at ~32 kDa can be observed. Use appropriate mitochondrial extraction or lysis buffers (e.g., RIPA with protease inhibitors) to ensure efficient solubilization of this integral membrane protein.
• In immunofluorescence/ICC applications, consider permeabilization with Triton X-100 or digitonin and validate mitochondrial co-localization with markers such as MitoTracker or anti‑COX IV.
• For ELISA, the antibody can be used for capture or detection of SDHB in biological fluids or cell lysates; optimize coating conditions and antibody concentrations for your specific assay.
• As with all polyclonal antibodies, lot-to-lot variability may occur; we recommend titrating the antibody in your system and including appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing hub for diagnostic manufacturers and laboratories, offering one-stop access to IVD raw materials from early research to clinical application. The SDHB rabbit polyclonal antibody is an excellent tool for investigating mitochondrial function, metabolic disorders, and oxidative stress pathways. Beyond this antibody, CamelBio provides validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare and niche targets to accelerate your IVD development pipeline.