Anti-SDHAF2 Polyclonal Antibody for WB, ELISA - Q9NX18

Core Product Specifications and Parameters

Biological Background: SDHAF2 Function and Localization

• Alternative names include PGL2, SDH5, hSDH5, and C11orf79. Encodes succinate dehydrogenase assembly factor 2, a mitochondrial chaperone.
• Essential for the assembly of succinate dehydrogenase (SDH), also known as respiratory complex II, which links the TCA cycle and mitochondrial electron transport chain.
• Required for flavinylation (covalent attachment of FAD) of the SDHA subunit, enabling full catalytic activity of the SDH complex.
• Localizes to the mitochondrial matrix.
• Classified as a chaperone and is involved in protein complex assembly.
• Associated with disease variants, including hereditary paraganglioma.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-166), making it suitable for detecting endogenous SDHAF2.
• Validated for Western blot (WB) and ELISA; consider optimizing antibody concentration for specific sample types.
• Cross-reactivity with mouse has been demonstrated; confirm reactivity in other species by sequence alignment.
• Use knockdown or knockout controls to verify band specificity in WB experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. Our portfolio includes validated antibody pairs, optimized polyclonal/monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. For mitochondrial metabolism and respiratory complex research, we offer a range of antibodies and supporting reagents to accelerate your assay development and validation workflows.