Anti-SDHA Monoclonal Antibody for WB, IF, ELISA - P31040

Core Product Specifications and Parameters

Biological Background: SDHA Function and Localization

• SDHA encodes the flavoprotein subunit of succinate dehydrogenase (SDH), an enzyme complex (complex II) of the mitochondrial electron transport chain that couples succinate oxidation to ubiquinone reduction (PubMed:10746566, PubMed:24781757).
  Related references:
  PMID:10746566 PMID:24781757

• The protein also catalyzes the oxidation of malate to enol-oxaloacetate, a potent SDH inhibitor that is subsequently isomerized to keto-oxaloacetate (By similarity).

• SDHA acts as a tumor suppressor; loss-of-function mutations are associated with paragangliomas and gastrointestinal stromal tumors (PubMed:20484225).
  Related references:
  PMID:20484225

• Localized to the mitochondrial inner membrane, SDHA is essential for both the tricarboxylic acid cycle and oxidative phosphorylation.

• Post-translational modifications include acetylation and phosphorylation, which may regulate enzyme activity; the protein also contains a covalently bound FAD cofactor.

• Clinically, SDHA mutations are linked to Leigh syndrome, cardiomyopathy, and other primary mitochondrial diseases (keywords: Disease variant, Primary mitochondrial disease).

• Multiple transcript variants encoding different isoforms have been identified (Alternative splicing).

• The protein has a predicted molecular mass of 72.7 kDa but commonly migrates at ~73 kDa on SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved C-terminal region (aa 550–650), suggesting recognition of all known human SDHA isoforms; verify in relevant sample systems.

• For Western blot, use reducing conditions and load appropriate protein amounts; the expected band is approximately 73 kDa. Validate with a positive control such as HeLa or HEK293 cell lysates.

• In immunofluorescence assays, mitochondrial staining should predominate; optimize fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) for best signal.

• This antibody cross-reacts with mouse and rat SDHA, making it suitable for studies in these species; confirm reactivity in your model by testing lysates or tissue sections.

• For ELISA applications, consider pairing with a detection antibody that recognizes a non-overlapping epitope.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers, laboratories, and research institutes with reliable access to premium IVD raw materials and technical expertise. As a one-stop sourcing partner, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare-target development—all designed to accelerate your SDHA-related diagnostic and research programs. Let CamelBio bridge your workflow from concept to clinic with efficient supply solutions tailored to your needs.