Anti-SDF4 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9BRK5

Core Product Specifications and Parameters

Biological Background: SDF4 Function and Localization

• SDF4 (stromal cell-derived factor 4), also known as Cab45 or SDF-4, is a 45 kDa calcium-binding protein with multiple isoforms produced by alternative splicing.
• The protein may regulate calcium-dependent activities in the endoplasmic reticulum lumen or a post-ER compartment, consistent with its localization to the Golgi apparatus lumen and cytoplasm.
• Isoform 5 appears specifically involved in the exocytosis of zymogens by pancreatic acini, highlighting a tissue-specific role in secretion.
• SDF4 is detected at the cell membrane and in bleb-like protrusions, suggesting dynamic subcellular trafficking.
• Ubiquitously expressed, with the pancreas primarily expressing isoform 5; other tissues predominantly express the canonical isoforms.
• Post-translational modifications include glycosylation (glycoprotein) and phosphorylation, as identified through proteomics.
• The protein belongs to a family characterized by calcium-binding EF-hand repeats, linking it to metal-ion-mediated signaling and homeostasis.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 37–200 of human SDF4 (NP_057260.2), which may encompass regions conserved across isoforms; verify isoform specificity in your experimental context.
• For Western blotting (WB), SDF4 has a predicted molecular weight of ~42 kDa (human); ensure appropriate gel percentage and transfer conditions for optimal detection.
• In immunohistochemistry-paraffin (IHC-P), consider antigen retrieval protocols and validate staining specificity in tissues with known SDF4 expression, such as pancreas for isoform 5.
• ELISA applications may benefit from using a matched pair system: pair this polyclonal antibody with a monoclonal capture antibody for sandwich ELISA development.
• Cross-reactivity is confirmed for human and mouse, but amino acid sequence alignment is advisable before applying to other species.
• Ideal experimental controls include SDF4 knockout or knockdown cell lines and tissue lysates to confirm band identity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to supporting diagnostic manufacturers and research institutes with a comprehensive portfolio of IVD raw materials. This anti-SDF4 polyclonal antibody is part of our collection covering key research targets for metabolic, secretion, and Golgi biology studies. Beyond individual antibodies, we provide validated antibody pairs, bulk ancillary reagents, and custom raw-material sourcing to streamline your assay development from concept to clinic.