Anti-SDC4 Rabbit Polyclonal Antibody for WB, ELISA - P31431

Core Product Specifications and Parameters

Biological Background: SDC4 Function and Localization

• SDC4 (amphiglycan/ryudocan core protein) is a cell surface heparan sulfate proteoglycan that regulates exosome biogenesis in collaboration with SDCBP and PDCD6IP.
  Related references: PMID:22660413
• The SDC4 gene undergoes alternative splicing, producing multiple transcript variants.
• The protein is extensively modified by heparan sulfate glycosaminoglycan chains, classifying it as a proteoglycan and glycoprotein.
• The predicted molecular weight of the unmodified core protein is 21.6 kDa (UniProt); the mature, glycosylated form is observed at approximately 22 kDa.
• SDC4 predominantly localizes to the plasma membrane and can also be secreted.
• SDC4 is expressed in fibroblasts and epithelial cells at the protein level.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular region (amino acids 19-145) of human SDC4, likely generating polyclonal antibodies that recognize both native and denatured protein.
• For Western blotting, anticipate a band at ~22 kDa; include appropriate positive and negative controls to confirm specificity.
• Suitable for ELISA applications; optimize coating conditions and antibody dilution according to your sample's target concentration.
• Cross-reactivity with species other than human should be tested experimentally if required for your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research institutions with validated antibody pairs, high-quality monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-SDC4 polyclonal antibody targets a key player in exosome biogenesis, supporting studies in cell communication and biomarker discovery. Leverage our integrated sourcing services to streamline your development pipeline from concept to clinical application.