Anti-SDC2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P34741

Core Product Specifications and Parameters

Biological Background: SDC2 Function and Localization

• Syndecan-2 (SDC2), also known as fibroglycan or HSPG, is a cell surface proteoglycan encoded by the SDC2 gene.
• It functions as a heparan sulfate proteoglycan involved in regulating dendritic arbor morphogenesis, a critical process in neuronal development.
• SDC2 is a member of the syndecan family of transmembrane proteoglycans, which act as co-receptors for various ligands and extracellular matrix components.
• The protein localizes to the plasma membrane and contains a single transmembrane domain, with extracellular heparan sulfate chains and a cytoplasmic tail subject to phosphorylation.
• SDC2 is implicated in cell differentiation and neurogenesis, contributing to cell signaling and adhesion processes.
• The unmodified core protein has a calculated molecular weight of approximately 22 kDa, but post-translational modifications with glycosaminoglycan chains increase the apparent mass.

Experimental Guidance and Technical Tips

• For Western blot (WB), the core SDC2 protein has a predicted molecular weight of ~22 kDa; however, extensive glycosylation with heparan sulfate chains often results in higher observed bands (e.g., >40 kDa). Consider using positive controls such as human, mouse, or rat cell lines known to express SDC2, and evaluate deglycosylation treatments to confirm specificity.
• In immunohistochemistry-paraffin (IHC-P), SDC2 exhibits membranous staining. Optimize antigen retrieval and antibody dilution for each tissue type; tissues with high SDC2 expression, such as brain, can serve as positive controls.
• For ELISA applications, the antibody may be used as either capture or detection reagent. Validate cross-species reactivity with mouse and rat samples, and include appropriate controls to minimize matrix effects.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic manufacturers and research groups by offering integrated access to IVD raw materials, including this SDC2 antibody for applications in cell signaling, neurogenesis, and heparan sulfate biology. Our portfolio covers validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets—bridging every step from concept to clinic.