Anti-SD1 Polyclonal Antibody for WB and ELISA - B6F2D9

Core Product Specifications and Parameters

Biological Background: SD1 Function and Localization

• The SD1 protein, encoded by the C20ox2 gene (also known as OsGA20ox2, SD1-2E, GA20, Sd-1, 20ox2, GA20OX2, Os20ox2, osGA20ox2), functions as a gibberellin 20-oxidase in Oryza sativa subsp. japonica.
• It is classified as an oxidoreductase that requires iron as a metal cofactor for enzymatic activity, as indicated by UniProt keywords.
• The calculated molecular weight is approximately 42.5 kDa (42513 Da), aligning with the observed band of 43 kDa in experimental applications.
• As a gibberellin 20-oxidase, it catalyzes a key step in gibberellin biosynthesis, contributing to plant growth and development regulation.
• Multiple aliases reflect functional roles and genetic variants, including sd1, GA20, Sd-1, 20ox2, C20ox2, SD1-2E, GA20OX2, Os20ox2, osGA20ox2, and SD1.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fusion protein spanning amino acids 1–140 of SD1 (accession Q0JH50). This N-terminal region may enable detection of multiple isoforms or closely related proteins in Oryza sativa.
• For Western blot, consider using rice tissue extracts and expect a band near 43 kDa. Optimal loading amounts and detection conditions should be empirically determined.
• In ELISA, this polyclonal antibody can be employed for direct or indirect detection; however, antibody dilutions must be optimized for each specific assay format.
• As the antibody is raised in rabbit, appropriate anti-rabbit secondary reagents are required. Cross-reactivity is specified for Oryza sativa only; application in other species should be validated independently.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for IVD manufacturers and research institutions, offering a streamlined source for high-quality raw materials. The SD1 antibody supports studies in plant gibberellin signaling and crop improvement, and CamelBio provides additional reagents such as recombinant proteins, antibody pairs, and bulk ancillary products to facilitate comprehensive assay development. Leverage our expertise to source rare targets and accelerate your diagnostic pipeline.