Anti-SCTR Rabbit Polyclonal Antibody - P47872

Core Product Specifications and Parameters

Biological Background: SCTR Function and Localization

• SCTR (secretin receptor) is a class B G protein-coupled receptor activated by the peptide hormone secretin, playing critical roles in digestion, energy balance, and fluid homeostasis.
• Upon secretin binding, SCTR activates G proteins and stimulates the cAMP-dependent signaling pathway, regulating duodenal pH by inhibiting gastric acid secretion and promoting pancreatic bicarbonate production (PubMed:32811827, PubMed:33008599). Related references: PMID:32811827 PMID:33008599
• SCTR mediates diet-induced thermogenesis by activating brown adipose tissue (BAT) lipolysis and thermogenesis, which promotes satiation (PubMed:25332973). Related references: PMID:25332973
• In the kidney, SCTR regulates renal water reabsorption, contributing to cellular osmoregulation.
• SCTR is primarily localized to the cell membrane, with enrichment at the basolateral membrane in polarized epithelial cells.
• The receptor undergoes post-translational modifications including N-linked glycosylation and phosphorylation, and contains conserved disulfide bonds important for structural stability.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 151-461 of human SCTR (P47872), covering a significant portion of the extracellular and transmembrane domains. Consider this broad epitope coverage when interpreting band patterns.
• For western blot (WB), the expected molecular weight is approximately 50–55 kDa (glycosylated form). Use membrane protein extraction protocols for optimal detection.
• Cross-reactivity with mouse SCTR is reported; validate antibody performance in the relevant sample system before quantitative studies.
• For ELISA applications, consider pairing with a detection antibody recognizing a non-overlapping epitope if a sandwich format is desired.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories across the entire development continuum. This polyclonal antibody against SCTR addresses emerging research needs in metabolic and gastrointestinal biology. Through our streamlined supply chain, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert sourcing for rare targets, empowering you to accelerate your diagnostic and research programs with confidence.