Anti-SCP2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P22307

Core Product Specifications and Parameters

Biological Background: SCP2 Function and Localization

• SCP2 encodes isoforms SCP2 (sterol carrier protein 2) and SCPx (sterol carrier protein X/SCP-X), also known as non-specific lipid-transfer protein (NSL-TP), acetyl-CoA C-myristoyltransferase, and propanoyl-CoA C-acyltransferase.
• It catalyzes the last step of peroxisomal beta-oxidation of branched-chain fatty acids and bile acid intermediates, and is active with medium and long straight chain 3-oxoacyl-CoAs. Related references: PMID:10706581
• SCP2 mediates the transfer of phospholipids, cholesterol, and gangliosides between membranes, and stimulates the conversion of 7-dehydrocholesterol to cholesterol, thereby playing a role in regulating steroidogenesis. Related references: PMID:17157249 PMID:7642518 PMID:8300590
• The protein localizes to peroxisomes, cytoplasm, mitochondria, and endoplasmic reticulum, consistent with its roles in lipid metabolism and transport.
• SCP2 is expressed predominantly in liver, fibroblasts, and placenta.
• Post-translational modifications include acetylation and phosphorylation, and the protein is identified in direct protein sequencing and proteomics identification studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length SCP2 isoform (1-322), making this antibody suitable for detecting both SCP2 and potentially SCPx if the epitope is conserved; however, isoform-specific validation is recommended.
• For Western blot (WB), the predicted molecular weight is 59 kDa (full-length, possibly SCPx), but SCP2 isoform may appear smaller; validate band sizes in the relevant sample system.
• For immunohistochemistry (IHC-P), consider antigen retrieval methods and include peroxisome-rich tissues (e.g., liver) as positive controls.
• For ELISA, ensure the coating antigen matches the immunogen region and optimize the antibody dilution.
• Since the antibody cross-reacts with mouse, it can be used for comparative studies in murine models; however, confirm specificity in genetically modified controls if possible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our SCP2 antibody supports research into peroxisomal fatty acid oxidation and lipid transfer, providing a reliable tool for metabolic and cardiovascular studies. From concept to clinic, CamelBio streamlines your diagnostic development with comprehensive technical services and consulting.