Anti-SCO2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O43819

Core Product Specifications and Parameters

Biological Background: SCO2 Function and Localization

• SCO2 is a copper metallochaperone essential for the synthesis and maturation of cytochrome c oxidase subunit II (MT-CO2/COX2). Together with SCO1, it facilitates the incorporation of copper into the Cu(A) site of MT-CO2/COX2. Related references: PMID:15229189 PMID:17189203 PMID:19336478
• SCO2 may also act as a thiol-disulfide oxidoreductase, regulating the redox state of SCO1 cysteines during MT-CO2/COX2 maturation. Related references: PMID:19336478
• It localizes to the mitochondrial inner membrane, consistent with its pivotal role in oxidative phosphorylation.
• Ubiquitously expressed across human tissues, reflecting its fundamental importance in cellular respiration.
• Belongs to the SCO1/2 family of metallochaperones; mutations in SCO2 are linked to mitochondrial disorders such as Leigh syndrome and cardiomyopathy.
• The protein possesses a transmembrane helix and transit peptide for mitochondrial import, with an apparent molecular weight of approximately 30 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100–266 of human SCO2; consider performing sequence alignment to confirm epitope conservation across species when using non-human samples.
• For Western blot, endogenous SCO2 runs near 30 kDa; enriched mitochondrial fractions or whole-cell lysates from human, mouse, or rat cells are suitable starting materials.
• When performing IHC-P, validate antibody specificity in target tissues by comparing staining patterns with known expression profiles or using appropriate controls.
• For ELISA, the antibody can be tested as a capture or detection reagent; optimal pairing should be determined experimentally with the analyte of interest.
• Because SCO2 is a mitochondrial protein, sample preparation (e.g., inclusion of protease inhibitors, brief sonication) may improve detection of the target.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a reliable channel to source IVD-grade raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents. For projects targeting mitochondrial disorders or copper metabolism pathways, we can assist in procuring anti-SCO2 antibodies and related reagents in bulk, along with tailored technical consultation to accelerate assay development. Our integrated supply model helps you bridge the gap from early-stage research to clinical-grade production.