Anti-SCNN1G Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P51170

Core Product Specifications and Parameters

Biological Background: SCNN1G Function and Localization

• SCNN1G encodes the gamma subunit of the heterotrimeric epithelial sodium channel (ENaC), which also contains alpha and beta pore-forming subunits.
• ENaC mediates electrodiffusion of sodium ions across the apical membrane of epithelial cells, with water following osmotically, serving as a key regulator of sodium and fluid homeostasis. Related references: PMID:24124190
• In the kidney, ENaC drives electrogenic sodium reabsorption, directly influencing blood pressure and overall electrolyte balance. Related references: PMID:12107247, PMID:7550319, PMID:8640238
• The channel is essential for maintaining airway surface liquid volume and composition, which is critical for effective mucociliary clearance. Related references: PMID:18507830, PMID:19017867, PMID:24124190
• Subcellularly, ENaC localizes to the apical cell membrane of polarized epithelial tissues.
• At the protein level, SCNN1G expression is predominantly observed in the kidney.
• Post-translational modifications including glycosylation, phosphorylation, and disulfide bond formation regulate channel activity and surface trafficking.
• Genetic variants in SCNN1G are clinically associated with pseudohypoaldosteronism type 1 (PHA1), bronchiectasis with or without elevated sweat chloride 3 (BESC3), and Liddle syndrome (LDLS2), underscoring its role in sodium balance disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100-250 of human SCNN1G, targeting an extracellular/intracellular domain; the antibody is expected to recognize the full-length protein at approximately 74 kDa in reducing SDS-PAGE.
• For Western blot, use a positive control such as human kidney lysate or ENaC-expressing cell lines; non-specific bands may appear due to polyclonal nature.
• In immunoprecipitation-based applications (IF-P) and immunohistochemistry (IHC-P), optimize permeabilization and antigen retrieval conditions; signal is anticipated at the apical membrane of epithelial cells.
• For ELISA, the antibody can be explored as a capture or detection reagent; pairing with a validated matched antibody is recommended for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with comprehensive access to IVD raw materials for ion channel and epithelial transport targets. We offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to streamline assay development. For SCNN1G and other ENaC subunits, our sourcing network can quickly locate rare-target raw materials to support your project from concept to clinic.