Anti-SCNN1G Polyclonal Antibody for WB, IHC-P, ELISA - P51170

Core Product Specifications and Parameters

Biological Background: SCNN1G Function and Localization

• SCNN1G encodes the gamma subunit of the heterotrimeric epithelial sodium channel (ENaC), a critical regulator of sodium balance and fluid homeostasis. Related references: PMID:30251954 PMID:32729833 PMID:7550319
• ENaC mediates the electrodiffusion of sodium ions across the apical membrane of epithelial cells, driving osmotic water reabsorption in the kidney and other tissues. Related references: PMID:24124190 PMID:12107247 PMID:8640238
• In the airways, ENaC activity is essential for maintaining airway surface liquid depth and proper mucus clearance. Related references: PMID:18507830 PMID:19017867 PMID:24124190
• The protein localizes predominantly to the apical cell membrane of epithelial cells.
• SCNN1G is primarily expressed in the kidney at the protein level, consistent with its major role in renal sodium handling.
• Post‑translational modifications include glycosylation, phosphorylation, disulfide bond formation, and ubiquitin conjugation, which regulate channel activity and stability.
• Mutations in SCNN1G are associated with pseudohypoaldosteronism type 1 (PHA1) and other disorders of sodium homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 85–355 of human SCNN1G; this region may allow detection of full-length and some processed forms of the channel.
• For Western blotting, a band around 74 kDa is expected under reducing conditions; validate expression in relevant kidney or epithelial cell lysates.
• When performing IHC‑P, antigen retrieval and titration of primary antibody concentration may be necessary; test in positive control tissues such as kidney.
• ELISA protocols can be developed using standard direct or indirect coating methods; consider pairing with a suitable detection antibody for sandwich assays.
• Cross‑reactivity with mouse SCNN1G is indicated; validate reactivity in mouse tissue lysates if switching species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs one-stop access to validated IVD raw materials, including polyclonal antibodies like this anti‑SCNN1G antibody, for renal physiology and ion‑channel research. Beyond single reagents, we supply optimized antibody pairs, bulk ancillary reagents, and can source rare targets to accelerate your product development pipeline. Contact us to discuss how our comprehensive portfolio can support your next project.