Anti-SCNN1B Polyclonal Antibody for WB and ELISA - P51168

Core Product Specifications and Parameters

Biological Background: SCNN1B Function and Localization

• SCNN1B (amiloride-sensitive sodium channel subunit beta, also known as ENaC beta) is one of three pore-forming subunits of the epithelial sodium channel (ENaC) heterotrimer (PubMed:30251954, PubMed:32729833, PubMed:7762608). Related references: PMID:30251954, PMID:32729833, PMID:7762608

• ENaC mediates electrodiffusion of sodium ions across the apical membrane of epithelial cells, driving osmotic water movement and regulating fluid homeostasis, especially in the kidneys (PubMed:12107247) and lungs (PubMed:24124190). Related references: PMID:12107247, PMID:24124190

• The channel is essential for airway surface liquid homeostasis, crucial for proper mucus clearance (PubMed:24124190). Related references: PMID:24124190

• SCNN1B is predominantly expressed in kidney, lung, and placenta (PubMed:7762608). At the protein level, it is detected in kidney (PubMed:22207244). Related references: PMID:7762608, PMID:22207244

• The protein localizes to the apical cell membrane and is also found in cytoplasmic vesicle membranes, indicating a role in membrane trafficking.

• Key post-translational modifications include N-linked glycosylation, phosphorylation, and disulfide bond formation, which are critical for channel activity and trafficking.

• Mutations in SCNN1B are associated with Liddle syndrome (gain-of-function) and pseudohypoaldosteronism type 1B (loss-of-function), highlighting its role in sodium balance disorders.

Experimental Guidance and Technical Tips

• The immunogen, corresponding to the C-terminal intracellular domain (aa 406-640), is predicted to provide stable epitope accessibility under denaturing conditions, making it suitable for Western blot (WB) analysis. For WB, consider using protein lysates from human or mouse kidney, lung, or placenta tissues where SCNN1B is expressed.
• For ELISA applications, optimization of coating antigen (e.g., recombinant protein or peptide) and detection conditions is recommended.
• The antibody has documented cross-reactivity with human and mouse SCNN1B; validate performance in your specific sample matrix and experimental setup.
• Because ENaC function depends on proper glycosylation and subunit assembly, ensure that sample preparation preserves these features if native state detection is required.
• Use appropriate positive and negative controls, such as SCNN1B-overexpressing cell lines and knockout models, to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For targets like SCNN1B in renal and epithelial ion transport research, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and support for rare target raw-material sourcing. Our integrated supply chain facilitates efficient procurement and customization to accelerate your assay development and manufacturing workflows.