Anti-SCN3B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NY72

Core Product Specifications and Parameters

Biological Background: Sodium channel regulatory subunit beta-3 (SCN3B) Function and Localization

• SCN3B (Sodium channel regulatory subunit beta-3) is an accessory subunit of voltage-gated sodium channels, modulating channel localization, gating kinetics, and cell surface expression. Related references: PMID:20558140, PMID:21051419
• It specifically modulates the activity of cardiac Nav1.5 (SCN5A) channels, influencing cardiac action potential propagation and excitability. Related references: PMID:20558140, PMID:21051419, PMID:24567321
• SCN3B also regulates Nav1.8 (SCN10A) by promoting oligomerization and accelerating recovery from inactivation. Related references: PMID:14975698
• It may interact with the atypical sodium channel Nav2.1 (SCN7A), suggesting a broader regulatory role. Related references: PMID:35301303
• The protein contains an N-terminal immunoglobulin-like domain, which is important for channel interactions, and features disulfide bonds and glycosylation sites.
• SCN3B is predominantly expressed in the atrium of the heart, and mutations are associated with atrial fibrillation and Brugada syndrome.
• Subcellular localization is at the cell membrane, where it complexes with pore-forming alpha subunits.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 23-160, which includes the extracellular Ig domain. This region is conserved across human, mouse, and rat, consistent with the antibody's cross-reactivity.
• For western blot (WB), a specific band at approximately 25 kDa is expected. Validate the band pattern in your sample system, as glycosylation may alter migration.
• For immunofluorescence (IF/ICC), expect membrane staining; optimize fixation and permeabilization conditions for your cell type.
• For ELISA, the antibody can be used to detect recombinant SCN3B or tissue lysates. Perform a standard curve with known concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop B2B sourcing partner for IVD raw materials, offering validated antibodies, antigen reagents, and customized solutions from concept to clinic. This anti-SCN3B polyclonal antibody supports ion channel and cardiac biomarker research, complementing our portfolio of polyclonal and monoclonal antibodies for voltage-gated sodium channels. Whether you need bulk ancillary reagents or rare target sourcing, CamelBio streamlines your supply chain with reliable, quality-controlled products.