Anti-SCN2A Polyclonal Antibody for WB, ELISA - Q99250

Core Product Specifications and Parameters

Biological Background: SCN2A Function and Localization

• SCN2A encodes the sodium channel protein type 2 subunit alpha (Nav1.2), a voltage-gated sodium channel subunit alpha also known as HBSC II, brain II, and type II sodium channel.
• The SCN2A-encoded Nav1.2 channel mediates voltage-dependent sodium ion permeability, opening in response to membrane depolarization to allow selective Na+ influx, crucial for action potential generation and propagation in neurons. Related references: PMID:1325650, PMID:17021166, PMID:28256214
• Nav1.2 is implicated in the regulation of hippocampal replay occurring within sharp wave ripples (SPW-R), a process important for memory consolidation.
• The protein localizes to the cell membrane, where it functions as a transmembrane ion channel.
• SCN2A undergoes alternative splicing, glycosylation, phosphorylation, and ubiquitination, which regulate channel expression and function.
• Mutations in SCN2A are associated with epileptic encephalopathies and other neurological disorders, making it a key disease-relevant target.

Experimental Guidance and Technical Tips

• The immunogen spans the intracellular loop between transmembrane domains S1 and S2 of domain I (amino acids 275-401), likely accessible in denaturing conditions; consider testing both reducing and non-reducing conditions for WB.
• For WB, the predicted molecular weight is 228 kDa; use low-percentage gels (e.g., 6-8%) and optimize transfer conditions for high-molecular-weight proteins.
• In ELISA, the polyclonal nature may provide signal amplification; validate with appropriate antigen controls and consider coating conditions.
• Lot-to-lot consistency should be verified for quantitative or critical applications, as inherent variability can occur with polyclonal antibodies.
• This antibody has been validated for human samples; cross-reactivity with other species has not been tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD-grade raw materials, including this anti-SCN2A polyclonal antibody for neurology and ion channel research. Our supply capabilities encompass bulk antibody production, validated antibody pairs, optimized monoclonal and polyclonal reagents, and rare-target sourcing. Partner with CamelBio to accelerate your project from concept to clinic with reliable, one-stop material procurement.