Anti-SCN1B Rabbit Polyclonal Antibody for WB, ELISA - Q07699

Core Product Specifications and Parameters

Biological Background: SCN1B Function and Localization

• SCN1B encodes the sodium channel regulatory subunit beta-1, an immunoglobulin domain-containing transmembrane protein that functions as an accessory subunit for voltage-gated sodium channels. It modulates the gating kinetics and membrane expression of Nav1.1, Nav1.2, Nav1.3, Nav1.4, Nav1.5, Nav1.6, Nav1.7, and Nav1.8. Related references: PMID:14622265 PMID:15525788 PMID:18464934
• In addition to its channel modulatory role, SCN1B functions as a cell adhesion molecule (CAM) critical for neuronal migration and pathfinding during brain development. It stimulates neurite outgrowth. Related references: PMID:21994374
• The protein exists as multiple isoforms due to alternative splicing. Isoform 1 is abundantly expressed in skeletal muscle, heart, and brain, while isoform 2 is highly expressed in brain and skeletal muscle with lower levels in other tissues. In the brain, isoform 2 predominates in the cerebellum, and isoform 1 is higher in the cerebral cortex.
• SCN1B localizes to the cell membrane, perikaryon, axon, and other cell projections, and can also be secreted. This widespread distribution supports both its ion channel regulatory and adhesive functions.
• Post-translational modifications include glycosylation and disulfide bonds, as indicated by keywords. The protein is associated with several human diseases, including epilepsy (DEE52, EIEE52, GEFSP1), atrial fibrillation (ATFB13), and Brugada syndrome (BRGDA5), underscoring its clinical importance.
• The calculated molecular weight of isoform 1 is approximately 24.7 kDa, while the immunogen-derived band is detected at 25 kDa in western blotting.

Experimental Guidance and Technical Tips

• For Western blotting, use standard reducing or non-reducing conditions as SCN1B contains disulfide bonds. Optimize blocking agents (e.g., 5% non-fat milk or BSA) and primary antibody dilution (start at 1:500–1:2000) using lysates from human, mouse, or rat brain, heart, or skeletal muscle tissues where the target is highly expressed.
• Due to the glycoprotein nature and potential post-translational modifications, a band shift may be observed; validate the detected band size (approximately 25 kDa) against the predicted molecular weight.
• For ELISA applications, the antibody can be used as a detection reagent; pair with a matched capture antibody for sandwich assays. Validate binding by testing recombinant SCN1B protein or tissue lysates.
• When studying isoform-specific expression, verify reactivity using isoform-specific positive controls or confirm epitope location within the immunogen sequence (aa 19–160) that is present in both major isoforms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to a broad portfolio of IVD raw materials, covering the full development continuum from concept to clinic. This SCN1B polyclonal antibody exemplifies our commitment to supplying high-quality, validated immunological reagents for neuroscience and cardiovascular biomarker research. Explore our offerings for bulk ancillary reagents, optimized monoclonal/polyclonal antibodies, and targeted sourcing of rare reagents to accelerate your assay development pipeline.