Anti-SCLY Polyclonal Antibody for WB, ELISA - Q96I15

Core Product Specifications and Parameters

Biological Background: SCLY Function and Localization

• SCLY (selenocysteine lyase), also known as SCL or hSCL, is a pyridoxal phosphate-dependent enzyme that catalyzes the decomposition of L-selenocysteine into L-alanine and elemental selenium.
• The enzyme plays a critical role in selenium recycling and the provision of selenium for selenoprotein biosynthesis.
• Subcellular localization studies indicate that SCLY resides predominantly in the cytoplasm, specifically the cytosol.
• Post-translational modifications, including acetylation and phosphorylation, have been identified and may regulate its enzymatic activity.
• Alternative splicing generates multiple transcript variants, potentially producing isoforms with distinct functional properties.
• Structural studies have resolved the 3D conformation of SCLY, revealing key residues involved in substrate binding and catalysis.
• UniProt keywords highlight its classification as a lyase and transferase, alongside evidence for proteomics identification and a reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 284–453), which is conserved among human, mouse, and rat, supporting cross-reactivity.
• For Western blot, the predicted molecular weight is 48 kDa; validate band specificity using lysates from relevant species and a suitable loading control.
• In ELISA, test the antibody against recombinant protein or endogenous antigen; optimize coating and detection conditions for your specific assay format.
• Since SCLY is cytosolic, consider subcellular fractionation to enrich the target and reduce background in WB experiments.
• Cross-adsorption or blocking with the immunogen may help confirm signal specificity if non-specific bands are observed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a complete range of IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This anti-SCLY polyclonal antibody is part of our offering for studies on selenium metabolism and selenoprotein function. Whatever your raw-material sourcing needs—common or rare targets—CamelBio provides integrated solutions and expert support to move your projects from concept to clinic.