Anti-SCIN Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y6U3

Core Product Specifications and Parameters

Biological Background: Scinderin (SCIN) Function and Localization

• Scinderin (also known as Adseverin) is a Ca²⁺-dependent actin filament-severing protein that regulates exocytosis by modulating the cortical actin network beneath the plasma membrane. Related references: PMID:26365202 PMID:8547642
• It also exhibits barbed end capping and nucleating activities in vitro, and its severing activity is inhibited by phosphatidylinositol 4,5-bisphosphate (PIP2).
• SCIN is required for megakaryocyte differentiation, maturation, polyploidization, and the release of platelet-like particles, and it inhibits cell proliferation and tumorigenesis through MAPK, p38, and JNK signaling pathways. Related references: PMID:11568009
• Subcellularly, SCIN localizes to the cytoplasm, cytoskeleton, and podosomes (cell projections).
• It is predominantly expressed in megakaryocytes.
• Key functional domains and features include actin capping, actin-binding, calcium/metal-binding, and phosphorylation sites; the protein contains multiple repeats.
• SCIN plays a role in osteoclastogenesis and actin cytoskeletal organization in osteoclasts, and regulates chondrocyte proliferation and differentiation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–100) of human SCIN; target detection may be optimized by using reducing and denaturing conditions for WB to resolve the predicted ~80 kDa band.
• For IF/ICC, fixation and permeabilization protocols compatible with cytoskeletal proteins are recommended; podosome-like structures may be visualized in relevant cell types.
• Cross-reactivity with mouse is reported; validate species-specific reactivity in your experimental system using appropriate controls.
• For ELISA, consider a sandwich format using this polyclonal antibody as a capture or detection reagent; pair with a monoclonal antibody for enhanced specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw material supplier, offering validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and laboratories. This anti-SCIN product supports research into cytoskeletal dynamics, megakaryocyte biology, and exocytosis, and we can assist with sourcing complementary raw materials for assay development. From concept to clinic, CamelBio bridges your procurement needs with reliable supply chains.