Anti-SCGB1A1 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P11684

Core Product Specifications and Parameters

Biological Background: SCGB1A1 Function and Localization

• SCGB1A1, also known as Uteroglobin, Club cell secretory protein (CC10), or Secretoglobin family 1A member 1, is a small secreted protein predominantly expressed in the nonciliated Club cells of the pulmonary airway epithelium.
• The protein binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCBs), and weakly progesterone, and acts as a potent inhibitor of phospholipase A2, suggesting a role in anti-inflammatory and surfactant homeostasis processes.
• SCGB1A1 is a member of the secretoglobin superfamily and has a molecular weight of approximately 10 kDa (theoretical 9994 Da), with a characteristic disulfide bond essential for its structure.
• It is a secreted protein, consistent with its function in the pulmonary airway lining fluid, and has been detected in urine (hence the alias UP-1).
• Post-translational modifications and biological significance of its direct protein sequencing are noted in reference proteomes.
• Tissue specificity is restricted to Club cells, making it a classical marker for nonciliated bronchiolar epithelial cells in lung biology.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 22-91 of human SCGB1A1, which corresponds to the mature secreted form; this region is likely critical for phospholipid and PCB binding. Consider verifying sequence homology with mouse and rat orthologs to confirm expected cross-reactivity.
• For Western blot, a specific band around 10 kDa should be detected in reducing conditions; use lung tissue or Club cell lysates as positive controls and validate with a knockdown or knockout sample where possible.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF-P), optimal results are expected in formalin-fixed paraffin-embedded lung sections, with staining localized to the cytoplasm of Club cells; include appropriate enzyme retrieval and blocking steps to minimize background.
• For ELISA, the antibody may be employed in sandwich or direct detection formats; pairing with a matched capture antibody against a distinct epitope could improve sensitivity for native SCGB1A1 in bronchoalveolar lavage fluid or serum.
• Cross-reactivity with mouse and rat has been reported; however, experimental validation in the relevant species and tissue type is recommended before large-scale studies.

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