Anti-SCG2 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P13521

Core Product Specifications and Parameters

Biological Background: Secretogranin-2 Function and Localization

• Secretogranin-2 (SCG2), also designated Chromogranin-C or Secretogranin II (SgII), is a member of the granin family of neuroendocrine secretory proteins.
• It functions in the regulation of secretory granule biogenesis, including the sorting of cargo and formation of dense-core vesicles.
• SCG2 is a secreted protein localized to secretory granules and is released upon stimulation.
• The protein undergoes post-translational modifications such as glycosylation, phosphorylation, sulfation, and proteolytic cleavage at paired basic residues.
• SCG2 contains calcium-binding domains, which contribute to its role in granule stability and aggregation.

Experimental Guidance and Technical Tips

• This polyclonal antibody, generated against amino acids 30-320, may recognize both full-length and processed forms of SCG2; include appropriate controls in your assays.
• For Western blotting, the predicted molecular weight of full-length SCG2 is approximately 71 kDa; additional bands from cleavage products (e.g., EM66) may be observed depending on the sample.
• In immunofluorescence (IF-P), intracellular detection of SCG2 in secretory granules requires cell permeabilization.
• The antibody has been tested in ELISA and can be used for quantitative detection of SCG2 in cell supernatants or lysates.
• Given cross-reactivity with rat, validate the antibody in rat-derived samples using positive control tissues such as pituitary or neuroendocrine cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a reliable partner for IVD manufacturers and researchers, offering a comprehensive portfolio of raw materials for neuroendocrine biomarker assays. We supply validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline your diagnostic development. Our sourcing capabilities also cover rare targets like SCG2, supporting your projects from early R&D to clinical application.