Anti-SCFD1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8WVM8

Core Product Specifications and Parameters

Biological Background: SCFD1 Function and Localization

• SCFD1 (Sec1 family domain-containing protein 1), also known as SLY1, is a member of the Sec1/Munc18 (SM) protein family, essential for SNARE-mediated membrane fusion events.
• Plays a critical role in SNARE-pin assembly, facilitating the docking and fusion of transport vesicles, specifically in Golgi-to-endoplasmic reticulum (ER) retrograde transport, via its interaction with the COG4 subunit of the conserved oligomeric Golgi (COG) complex.
• Involved in the broader regulation of vesicular transport between the ER and the Golgi apparatus, ensuring proper protein trafficking and organelle homeostasis.
• Localizes to the cytoplasm, endoplasmic reticulum membrane, and Golgi apparatus membrane, in particular the Golgi stack membrane, consistent with its function in ER-Golgi trafficking.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity or interactions within the secretory pathway.
• The gene undergoes alternative splicing, potentially generating isoforms with distinct functional properties.
• Keywords: Acetylation, Alternative splicing, Cytoplasm, Direct protein sequencing, Endoplasmic reticulum, ER-Golgi transport, Golgi apparatus, Membrane, Phosphoprotein, Protein transport, Proteomics identification, Reference proteome, Transport.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein corresponding to amino acids 373-642 of human SCFD1, covering a region likely to be well-conserved across species, which supports the observed cross-reactivity with mouse and rat.
• For Western blot (WB), a band at approximately 72 kDa is expected; loading controls and molecular weight markers should be used to confirm specificity.
• In immunohistochemistry (IHC-P), optimization of antigen retrieval conditions (e.g., heat-induced epitope retrieval in citrate buffer, pH 6.0) may enhance staining; validate specificity by comparing with known expression patterns or using blocking peptides.
• For ELISA applications, consider direct or capture formats and validate using recombinant SCFD1 protein or cell lysates to establish sensitivity and dynamic range.
• As a polyclonal antibody, lot-to-lot variability is possible; we recommend including positive (e.g., HeLa cell lysate) and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to validated IVD raw materials, including this anti-SCFD1 polyclonal antibody for ER-Golgi transport research. As your strategic sourcing partner, we offer matched antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary reagents, and support for rare target raw-material sourcing—streamlining your path from concept to clinic.