Anti-SCD5 Rabbit Polyclonal Antibody for WB, ELISA - Q86SK9

Core Product Specifications and Parameters

Biological Background: SCD5 Function and Localization

• SCD5 encodes stearoyl-CoA desaturase 5, an enzyme that introduces the first double bond into saturated fatty acyl-CoA substrates, catalyzing the insertion of a cis double bond at the delta-9 position. Related references: PMID:15610069, PMID:15907797, PMID:22745828
• It generates a mixture of 16:1 and 18:1 unsaturated fatty acids. Related references: PMID:15610069, PMID:15907797
• SCD5 is involved in neuronal cell proliferation and differentiation through down-regulation of EGFR/AKT/MAPK and Wnt signaling pathways. Related references: PMID:22745828
• The protein localizes to the endoplasmic reticulum membrane.
• It is expressed in fetal brain, adult brain, pancreas, and at lower levels in kidney and lung; also detected in spiral ganglion cells and organ of Corti of fetal cochlea. Related references: PMID:31972369
• SCD5 belongs to the fatty acid desaturase family and is also known as SCD4, ACOD4, FADS4, HSCD5, DFNA79.
• Mutations in SCD5 are associated with non-syndromic deafness (DFNA79), and the protein may play a role in hearing.
• It requires iron as a cofactor and contains transmembrane helices.

Experimental Guidance and Technical Tips

• The immunogen was designed from a synthetic peptide spanning amino acids 231-330 of human SCD5, which may provide target specificity; consider validating in the relevant sample system.
• For Western blot, the predicted molecular weight is 38 kDa; include appropriate positive controls (e.g., human, mouse, or rat tissue lysates) and optimize loading amounts to account for species-specific expression levels.
• In ELISA assays, titrate the antibody and use suitable coating buffers; cross-reactivity with mouse and rat SCD5 may be exploited for multi-species studies.
• Due to alternative splicing and potential isoforms, confirm band patterns with reference samples and consider additional validation methods if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials. Our SCD5 polyclonal antibody supports studies in fatty acid metabolism, neuronal differentiation, and hearing function. As your sourcing bridge from concept to clinic, we offer validated antibody pairs, bulk ancillary reagents, and tailored raw-material solutions to accelerate your diagnostic development.