Anti-SCD1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O00767

Core Product Specifications and Parameters

Biological Background: SCD1 Function and Localization

• SCD1, also known as acyl-CoA desaturase or delta-9 desaturase, is a key enzyme in fatty acid metabolism encoded by the SCD gene.
• Catalyzes the insertion of a cis double bond at the delta-9 position of palmitoyl-CoA and stearoyl-CoA, utilizing O2 and cytochrome b5 electrons, to produce 16:1 and 18:1 monounsaturated fatty acids. Related references: PMID:15907797 PMID:18765284 PMID:15610069
• Plays a critical role in lipid biosynthesis, contributing to membrane phospholipids, cholesterol esters, and triglycerides, and regulating lipogenic gene expression and mitochondrial fatty acid oxidation. Important for body energy homeostasis.
• Localized to the endoplasmic reticulum membrane.
• Highly expressed in adult adipose tissue; detected in fetal liver, lung, and brain, and at lower levels in adult brain and lung.
• Post-translational modifications include phosphorylation; identified by direct protein sequencing.
• Contains iron and other metal-binding sites; features a transmembrane helix and belongs to the fatty acid desaturase family.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a band around 42 kDa. Validate in lysates from adipose tissue or liver, where SCD is highly expressed.
• In immunohistochemistry (paraffin), optimize antigen retrieval; positive staining is anticipated in adipose and hepatic tissues.
• For immunofluorescence/ICC, permeabilization is necessary to detect the ER-membrane localized SCD protein; include appropriate controls.
• In ELISA applications, consider using recombinant SCD or the immunizing peptide as a positive control to confirm binding.
• Cross-reactivity with mouse and rat suggests the epitope is conserved; this may facilitate translational studies in rodent models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers by offering a comprehensive portfolio of IVD raw materials and technical services. This anti-SCD1 polyclonal antibody exemplifies the quality reagents available for lipid metabolism and metabolic research. Beyond antibodies, CamelBio provides validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to accelerate your IVD development from concept to clinic.