Anti-SCD Polyclonal Antibody for WB, IF/ICC, ELISA - O00767

Core Product Specifications and Parameters

Biological Background: Stearoyl-CoA desaturase (SCD) Function and Localization

• Stearoyl-CoA desaturase (SCD), also known as Delta-9 desaturase, catalyzes the insertion of a cis double bond at the delta-9 position of fatty acyl-CoA substrates, requiring molecular oxygen and electrons from reduced cytochrome b5. Related references: PMID:15907797, PMID:18765284
• The enzyme converts palmitoyl-CoA (16:0) and stearoyl-CoA (18:0) into palmitoleate (16:1) and oleate (18:1), respectively, generating a mixture of monounsaturated fatty acids. Related references: PMID:15610069
• SCD plays a central role in lipid biosynthesis, regulating lipogenic gene expression and mitochondrial fatty acid oxidation, thereby contributing to whole-body energy homeostasis.
• It is essential for the biosynthesis of membrane phospholipids, cholesterol esters, and triglycerides.
• Subcellularly, SCD localizes to the endoplasmic reticulum membrane as a multi-pass transmembrane protein.
• Tissue expression is highest in adult adipose tissue; moderate levels are found in fetal liver, lung, and brain, with lower expression in adult brain and lung.
• The protein contains iron-binding sites critical for its desaturase activity and is identified as a phosphoprotein in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from amino acids 260–359 of human SCD. For Western blot, a band at approximately 42 kDa is expected; consider using appropriate molecular weight markers.
• In immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization conditions to ensure detection of the ER-membrane-localized target.
• Validate the antibody in your specific sample system and assay conditions, as cross-reactivity and performance may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive range of IVD raw materials, from validated monoclonal/polyclonal antibodies to bulk ancillary reagents. For lipid metabolism studies, this anti-SCD polyclonal antibody is part of our portfolio of high-quality targets; we also offer one-stop access to optimized antibody pairs, rare target reagents, and technical consulting to streamline your development from concept to clinic.