Anti-SCAMP2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O15127

Core Product Specifications and Parameters

Biological Background: SCAMP2 Function and Localization

• SCAMP2 (Secretory carrier-associated membrane protein 2) is a transmembrane protein involved in post-Golgi recycling pathways.
• It functions as a recycling carrier, mediating transport from endosomes to the cell surface.
• SCAMP2 localizes to the trans-Golgi network membrane and recycling endosome membrane, consistent with its role in membrane trafficking.
• The protein is widely expressed across tissues, indicating a fundamental role in cellular transport.
• SCAMP2 contains transmembrane domains and is a phosphoprotein, suggesting regulation by phosphorylation.
• It belongs to the secretory carrier membrane protein (SCAMP) family, which are components of post-Golgi membranes.
• Keywords identify SCAMP2 in proteomics studies and as a reference proteome member, underscoring its biological significance.
• The calculated molecular weight is approximately 36.6 kDa, with an observed band around 37 kDa in Western blot.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1–152) corresponds to the N-terminal cytoplasmic domain; the antibody is likely to recognize denatured protein well in WB.
• For IF/ICC, permeabilization is essential to allow access to the cytoplasmic epitope; validate staining under optimized fixation/permeabilization conditions.
• In ELISA, the recombinant immunogen can serve as a positive control; consider testing against native SCAMP2 in relevant lysates.
• Given the reported cross-reactivity with mouse and rat, verify performance in your specific sample system if working with these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For studies in membrane trafficking and endosomal recycling, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your assay development. Count on CamelBio as your procurement partner to secure high-quality SCAMP2 reagents and beyond.