Anti-SCAMP2 Polyclonal Antibody for WB, ELISA - O15127

Core Product Specifications and Parameters

Biological Background: SCAMP2 Function and Localization

• SCAMP2 (Secretory Carrier-Associated Membrane Protein 2) is a widely expressed transmembrane protein with an observed molecular weight of approximately 37 kDa (predicted MW 36.6 kDa).
• It functions in post-Golgi recycling pathways, acting as a recycling carrier to transport cargo from the trans-Golgi network (TGN) to the cell surface.
• SCAMP2 localizes predominantly to the trans-Golgi network membrane and recycling endosomes, consistent with its role in endomembrane trafficking.
• Belonging to the SCAMP family, it possesses four transmembrane helices and a conserved cytoplasmic domain that likely mediate membrane integration and protein interactions.
• The protein is classified as a phosphoprotein, indicating potential regulation by phosphorylation events that could modulate its trafficking activity.
• Keywords associated with SCAMP2 include protein transport, endosome, and Golgi apparatus, underscoring its essential role in intracellular vesicle-mediated transport.
• It is identified as a reference proteome component through extensive proteomics studies, validating its broad cellular significance.
• SCAMP2 exhibits tissue-wide expression, suggesting a housekeeping function in membrane recycling across different cell types.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (aa 1–152) of human SCAMP2; therefore, the antibody is expected to recognize epitopes within this domain.
• For Western blotting, a band near 37 kDa is anticipated in human cell lysates; consider using positive controls such as HeLa or HEK293 cells and optimizing primary antibody dilutions.
• In ELISA applications, plate the recombinant antigen or coat with cell lysates; a serial dilution series of the antibody can help determine the optimal working concentration.
• As a polyclonal antibody, batch-to-batch affinity may vary; it is advisable to validate new lots in the intended assay system before large-scale use.
• The antibody is reported to react with human SCAMP2; cross-reactivity with other species is not indicated, so species-specific validation is recommended if working with non-human samples.
• Upon receipt, aliquot and store at -20°C or -80°C, avoiding repeated freeze-thaw cycles to preserve antibody integrity and activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, offering everything from validated antibody pairs and optimized polyclonals to bulk ancillary reagents. This SCAMP2 antibody supports research into membrane trafficking and endosomal recycling pathways, with potential utility in diagnostic assay development. Whether you need rare-target raw materials or technical consultation, CamelBio bridges your concept to clinic needs.