Anti-SCAF8 (RBM16) Rabbit Polyclonal Antibody for WB, ELISA - Q9UPN6

Core Product Specifications and Parameters

Biological Background: SCAF8 Function and Localization

• SCAF8 (SR-related and CTD-associated factor 8), also known as RBM16 or CCAP7, is a 141 kDa nuclear protein that functions as an anti-terminator during transcription.
• Together with SCAF4, it prevents early mRNA termination by suppressing the use of alternative polyadenylation sites, thereby ensuring full-length transcript synthesis. Related references: PMID:31104839
• SCAF8 associates with the phosphorylated C-terminal domain (CTD) of RNA polymerase II and binds nascent RNA upstream of early poly(A) sites to inhibit premature cleavage and polyadenylation. Related references: PMID:31104839
• The protein also acts as a positive regulator of transcript elongation independently of SCAF4. Related references: PMID:31104839
• Subcellularly, SCAF8 is localized to the nucleus and nuclear matrix, consistent with its role in co-transcriptional RNA processing.
• Post-translational modifications include phosphorylation and methylation, and the protein undergoes alternative splicing, isopeptide bond formation, and ubiquitin-like conjugation.
• It is a member of the RNA-binding motif protein family and contains domains for RNA binding and CTD interaction.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 572–634 of human SCAF8. This region is expected to be immunogenic; however, users should validate antibody specificity in their target species and sample type.
• For Western blotting, SCAF8 has a predicted molecular weight of 141 kDa. We recommend using a low-percentage polyacrylamide gel (e.g., 6–8%) and extended transfer times to ensure efficient transfer of this high-molecular-weight protein.
• In ELISA applications, optimization of coating antigen and antibody dilution is advised. Consider using recombinant SCAF8 or cell lysates as positive controls.
• This polyclonal antibody is reported to cross-react with mouse and rat SCAF8. Users should verify reactivity if using samples from other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic developers and researchers from concept to clinic. This SCAF8 rabbit polyclonal antibody exemplifies our ability to supply quality research reagents for transcription and RNA processing studies. In addition to primary antibodies, CamelBio offers validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets.