Anti-SCAF4 Rabbit Polyclonal Antibody for IHC-P and ELISA - O95104

Core Product Specifications and Parameters

Biological Background: SCAF4 Function and Localization

• SCAF4 (SR-related and CTD-associated factor 4), also known as SRA4 and SFRS15, is encoded by the SCAF4 gene (KIAA1172) in humans.
• Functions as an anti-terminator protein that prevents early mRNA termination during transcription by suppressing premature polyadenylation, together with SCAF8. Related references: PMID:31104839
• Associates with the phosphorylated C-terminal domain (CTD) of RNA polymerase II and binds nascent RNA upstream of early poly(A) sites to block cleavage and polyadenylation. Related references: PMID:31104839
• Independently of SCAF8, SCAF4 also acts as a suppressor of transcriptional readthrough. Related references: PMID:31104839
• Localizes to the nucleus.
• Subject to acetylation and phosphorylation; undergoes alternative splicing.
• Mutations in SCAF4 are associated with epilepsy and intellectual disability.
• Part of the reference proteome; possesses RNA-binding activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 170-248 of human SCAF4; sequence homology across human, mouse, and rat may support the observed cross-reactivity, but consider verifying reactivity and specificity in the relevant sample system.
• For IHC-P, optimal working dilutions must be determined empirically; antigen retrieval methods can significantly influence staining patterns and intensity.
• In ELISA applications, the recombinant immunogen or target protein can serve as a positive control to validate assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, offering validated antibodies like this anti-SCAF4 polyclonal antibody for transcription research. Our supply capabilities encompass optimized antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing, supporting your development from early-stage projects to clinical application.