Anti-SBF1 Rabbit Polyclonal Antibody for WB, ELISA - O95248

Core Product Specifications and Parameters

Biological Background: SBF1 Function and Localization

• SBF1/MTMR5 is an inactive phosphatidylinositol 3-phosphatase that functions as an adapter for the active phosphatase MTMR2, regulating its catalytic activity and subcellular localization.
  Related references:
  PMID:12668758
• Exhibits guanine nucleotide exchange factor (GEF) activity, promoting GDP/GTP exchange to activate RAB28 and other Rab proteins.
  Related references:
  PMID:20937701
• Functions as a suppressor of autophagy in neurons; together with MTMR2, it regulates phosphoinositide dephosphorylation required for autophagy initiation and autophagosome maturation.
  Related references:
  PMID:35580604
• Positively regulates late-stage radial sorting of large caliber axons, facilitating myelination by Schwann cells through endosomal trafficking modulation (By similarity).
• Inhibits myoblast differentiation and drives oncogenic transformation in fibroblasts.
  Related references:
  PMID:9537414
• Subcellular localization includes the cytoplasm, perinuclear region, and neuron projections, consistent with its role in neuronal trafficking.
• Predominantly expressed in neurons of the frontal cortex, deep cerebellar nuclei, and cerebellar molecular and granular layers.
• Mutations in SBF1 are linked to Charcot-Marie-Tooth disease type 4B3 (CMT4B3), a severe demyelinating peripheral neuropathy.

Experimental Guidance and Technical Tips

• The large molecular weight of SBF1 (208 kDa) requires low-percentage polyacrylamide gels and extended transfer times for effective western blot detection.
• The immunogen covers a C-terminal region (aa 1630–1770); consider verifying isoform specificity if multiple splicing variants are investigated.
• Cross-reactivity with rat is indicated; validate the antibody in the relevant species and tissue type (e.g., brain lysates) before critical experiments.
• For ELISA, the antibody can be used as a capture or detection reagent; optimal pairing should be determined empirically.
• As a polyclonal preparation, include appropriate negative controls and monitor lot-to-lot consistency in your assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic developers and research laboratories with a single entry point to high-quality IVD raw materials. This anti-SBF1 antibody complements our growing portfolio of neurodegeneration and autophagy markers, and we can source additional validated pairs, bulk ancillary reagents, and rare-target raw materials tailored to your assay pipeline. Contact us to streamline your discovery-to-clinic journey.