Anti-SAV1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9H4B6

Core Product Specifications and Parameters

Biological Background: SAV1 Function and Localization

• Protein salvador homolog 1 (SAV1), also known as 45 kDa WW domain protein (hWW45), is a key scaffolding component of the Hippo signaling pathway.
• SAV1 functions as a regulator of STK3/MST2 and STK4/MST1 kinases, forming a complex that phosphorylates and activates LATS1/2, leading to YAP1 inactivation. This cascade restricts proliferation and promotes apoptosis, playing a pivotal role in organ size control and tumor suppression. Related references: PMID:29063833
• It promotes cell-cycle exit and terminal differentiation in developing epithelial tissues.
• SAV1 regulates centrosome disjunction by modulating NEK2 localization and its phosphorylation of CROCC and CEP250.
• In conjunction with STK3/MST2, SAV1 can activate transcriptional activity of estrogen receptor alpha (ESR1) through phosphorylation modulation.
• The protein localizes to both the nucleus and cytoplasm, consistent with its regulatory roles in signaling transduction and gene expression.
• SAV1 is ubiquitously expressed in adult tissues, with highest levels in pancreas, aorta, and interventricular septum, and lower in skeletal muscle; it is also expressed in various cell lines.
• Post-translational modifications include phosphorylation, and the protein contains a coiled coil domain and WW repeats.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 199-383 of human SAV1, targeting the C-terminal region; this may provide specific recognition of full-length SAV1 and possibly some isoforms.
• For Western blotting, a band around 45 kDa is expected; consider optimizing loading controls and antibody dilution for your specific sample.
• In immunohistochemistry (paraffin-embedded), antigen retrieval conditions may need optimization; validate staining specificity using appropriate controls.
• ELISA can be used for quantitative detection; cross-reactivity with mouse and rat allows multi-species studies.
• Since SAV1 is a phosphoprotein, consider phosphatase inhibitor treatment during sample preparation to preserve phosphorylation states.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to high-quality IVD raw materials, supporting diagnostic manufacturers and research laboratories from discovery through clinical application. For the SAV1/Hippo pathway research area, we provide a range of validated antibodies, bulk reagents, and custom sourcing services to accelerate your assay development. Contact us to source monoclonal and polyclonal antibodies, ancillary reagents, and tailored technical support for your specific needs.