Anti-SATB2 Monoclonal Antibody for WB, IF-P, IHC-P, IP, ELISA - Q9UPW6

Core Product Specifications and Parameters

Biological Background: SATB2 Function and Localization

• SATB2 (Special AT-rich sequence-binding protein 2) is a DNA-binding protein that recognizes the sugar-phosphate backbone of double-stranded DNA and binds to nuclear matrix or scaffold attachment regions (MARs).
• It acts as a transcription factor and chromatin regulator, recruiting both corepressors (HDACs) and coactivators (HATs) to modulate local chromatin-loop remodeling at promoters and enhancers.
• Plays a critical role in cortical development by initiating upper-layer neuron (UL1) genetic programs and repressing deep-layer neuron genes, partly through regulation of BCL11B (Ctip2), thereby influencing corticocortical connectivity.
• Involved in skeletal development and osteoblast differentiation, serving as a molecular node in transcriptional networks governing bone formation.
• May also contribute to palate formation during embryogenesis.
• Localizes to the nuclear matrix, consistent with its role in chromatin organization and gene regulation.
• High expression in adult brain (especially amygdala, corpus callosum, caudate nucleus, hippocampus), moderate in fetal brain, and weak in adult liver, kidney, and spinal cord.
• Post-translationally modified by phosphorylation and ubiquitination; subject to alternative splicing and linked to chromosomal rearrangements implicated in intellectual disability and other diseases.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 634-733 (NP_001165980.1); consider verifying the sequence conservation in your species of interest when assessing cross-reactivity.
• For Western blotting (WB), a band at approximately 83 kDa is expected; optimize antibody dilution using a positive control such as human or mouse brain lysate.
• For immunofluorescence (IF) and immunohistochemistry (IHC), nuclear localization should be observed; include appropriate nuclear counterstaining and validate in relevant tissue sections, especially brain or bone.
• For immunoprecipitation (IP), consider using a cross-linking method if preserving protein-DNA interactions is desired, given SATB2’s chromatin-binding function.
• When using this antibody in ELISA, confirm specificity with a control peptide or recombinant protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research labs by providing integrated access to premium IVD raw materials—from validated antibody pairs to bulk ancillary reagents. For neuroscience and developmental biology applications targeting SATB2, we offer optimized monoclonal antibodies and support for rare target sourcing. Partner with us to streamline your assay development pipeline from concept to clinic.