Anti-SATB1 Rabbit Polyclonal Antibody for WB, IP, IF-P, IHC-P, ELISA - Q01826

Core Product Specifications and Parameters

Biological Background: SATB1 Function and Localization

• SATB1 (Special AT-rich sequence-binding protein 1) is a DNA-binding protein that specifically recognizes the sugar-phosphate backbone of double-stranded DNA at special AT-rich sequences, predominantly at nuclear matrix/scaffold attachment regions (MARs).
• Acts as a transcriptional repressor or activator by inducing local chromatin-loop remodeling and serving as a docking platform for chromatin-modifying enzymes such as histone acetyltransferases (HATs) and deacetylases (HDACs), thereby regulating gene expression.
• Plays a crucial role in T-cell development by modulating genes essential for the maturation of CD8 single-positive thymocytes.
• Mediates X chromosome inactivation during embryogenesis by acting as a silencing factor downstream of Xist RNA.
• Regulates globin gene switching during erythroid differentiation, controlling fetal and adult hemoglobin expression.
• Facilitates HIV-1 integration by creating a favored chromatin context at Alu-like motifs, and HIV-1 Tat protein can override SATB1-mediated repression of interleukin genes.
• Reprogrammes chromatin organization and transcription in breast tumors, promoting metastasis by upregulating metastasis-associated genes and downregulating tumor suppressors.
• Subcellularly localized to the nucleus, particularly at the nuclear matrix and PML bodies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 572–763), which may contain conserved epitopes; verify cross-reactivity in the desired species and cell types.
• For Western blotting, an expected band at approximately 86 kDa should be observed; use appropriate positive and negative controls.
• In immunofluorescence and IHC-P, consider antigen retrieval protocols optimized for nuclear proteins and include a DNA counterstain to confirm nuclear localization.
• For immunoprecipitation, validate the antibody with known interaction partners or a suitable negative IgG control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials and custom sourcing services, including high-quality antibodies like this SATB1 polyclonal antibody for chromatin and immunology research. Leverage our supply capabilities for validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your diagnostic development. With one-stop access from concept to clinic, CamelBio supports your entire workflow.