Anti-SATB1 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q01826

Core Product Specifications and Parameters

Biological Background: SATB1 Function and Localization

• SATB1 (Special AT-rich sequence-binding protein 1) is a DNA-binding protein that acts as a transcriptional repressor and chromatin organizer, binding to matrix attachment regions (MARs) via consensus SATB1-binding sequences (CSBS).
• It serves as a docking platform for chromatin-modifying enzymes, recruiting both corepressors (e.g., HDACs) and coactivators (e.g., HATs) to regulate gene expression, essential for T-cell maturation and globin gene switching during erythroid differentiation.
• SATB1 plays a critical role in X chromosome inactivation by acting as a silencing factor for Xist RNA-mediated initiation during embryogenesis and lymphoma.
• The protein localizes to the nuclear matrix, nucleus, and PML bodies, consistent with its role in higher-order chromatin architecture.
• It is involved in reprogramming chromatin organization and transcription profiles in breast tumors, upregulating metastasis-associated genes and downregulating tumor-suppressor genes, thereby promoting growth and metastasis.
• Functional interactions with viral components have been reported: SATB1 interacts with cytomegalovirus (CMV) unique region and HIV-1 integration machinery, while HIV-1 Tat overcomes SATB1-mediated repression of IL2 and IL2RA.
• Predominantly expressed in thymus, reflecting its key role in immune T-cell development.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (isopeptide bond), which regulate its repressor activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 572–763 of human SATB1, a region that may be conserved across species; cross-reactivity with mouse and rat should be validated in the relevant sample system.
• For Western blot (WB), detect SATB1 at approximately 86 kDa; consider using appropriate loading controls and confirm specificity with knockdown or knockout samples where available.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), expect nuclear staining with possible PML body localization; optimize fixation and permeabilization conditions to preserve nuclear architecture.
• ELISA applications may be developed for quantification of SATB1; consider pairing with a matched detection antibody or standard curve for accurate measurement.
• Given SATB1's role in chromatin remodeling and transcriptional regulation, consider functional assays (e.g., ChIP) to study DNA-binding activity, though this antibody's suitability for such applications should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-SATB1 monoclonal antibody supports research into chromatin biology, transcriptional regulation, and cancer metastasis. For customized requirements or rare target sourcing, CamelBio's integrated supply chain bridges the gap from concept to clinic.