Anti-SAT1 Rabbit Polyclonal Antibody for WB, ELISA - P21673

Core Product Specifications and Parameters

Biological Background: SAT1 Function and Localization

• SAT1 (spermidine/spermine N1-acetyltransferase 1), also known as diamine acetyltransferase 1, SSAT, or putrescine acetyltransferase, is a key enzyme in polyamine metabolism.
• It catalyzes the acetylation of polyamines, with substrate preference norspermidine = spermidine >> spermine > N(1)-acetylspermine. Related references: PMID:15283699 PMID:16455797 PMID:17516632
• SAT1 tightly regulates intracellular polyamine concentrations and is involved in polyamine transport out of cells. Related references: PMID:16455797
• The enzyme also acetylates 1,3-diaminopropane and 1,5-diaminopentane. Related references: PMID:16455797 PMID:17516632
• SAT1 localizes to the cytosol.
• It belongs to the acyltransferase family and its three-dimensional structure has been determined.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein corresponding to amino acids 1-171 of human SAT1, which may elicit polyclonal antibodies targeting multiple epitopes suitable for Western blot detection.
• For Western blot, a specific band around 20 kDa is expected; include positive and negative controls to confirm reactivity.
• When performing ELISA, consider optimizing coating antigen and antibody dilutions to achieve optimal signal.
• Although cross-reactivity with mouse is indicated, validate the antibody's performance in mouse tissue or cell lysates to ensure comparable sensitivity.
• To maintain stability, avoid repeated freeze-thaw cycles and store as specified upon arrival.

CamelBio: Your One-Stop Sourcing Bridge

For researchers and IVD manufacturers focusing on polyamine metabolism and cancer-related pathways, CamelBio provides reliable access to high-quality raw materials like this anti-SAT1 polyclonal antibody. As a dedicated one-stop sourcing platform, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support for rare target procurement—streamlining your journey from concept to clinic.