Anti-SART3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q15020

Core Product Specifications and Parameters

Biological Background: SART3 Function and Localization

• SART3 (Spliceosome associated factor 3), also known as SART-3, Tip110, or p110 nuclear RNA-binding protein, is a 110 kDa U6 snRNP-binding protein crucial for spliceosome recycling.
• Acts as a recycling factor for U4/U6 snRNPs, promoting the reassembly of U4 and U6 snRNPs after spliceosome disassembly, a key step in pre-mRNA splicing. Related references: PMID:12032085
• Also binds U6atac snRNPs and functions in the recycling of U4atac/U6atac snRNPs, facilitating U12-type spliceosome assembly for splicing non-canonical introns. Related references: PMID:14749385
• Serves as a substrate-targeting factor for deubiquitinases, recruiting USP4 to ubiquitinated PRPF3 in the U4/U5/U6 tri-snRNP, thereby regulating spliceosome disassembly. Related references: PMID:20595234
• Recruits USP15 to histone H2B, mediating histone deubiquitination and potentially impacting gene expression and DNA repair. Related references: PMID:24526689
• May play a role in hematopoiesis, possibly through transcriptional regulation of genes like MYC (By similarity).
• Subcellularly localizes to the nucleus, with enrichment in Cajal bodies and nuclear speckles, and is also detected in the cytoplasm.
• Subject to multiple post-translational modifications including acetylation, phosphorylation, and methylation, which may regulate its activity and interactions.
• Ubiquitously expressed across human tissues, consistent with its fundamental role in mRNA splicing.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment (aa 664-963), so the antibody may recognize full-length SART3 and any isoforms or degradation products containing this region. Validate specificity in your sample system.
• For Western blot (WB), the predicted molecular weight is ~110 kDa. Note that actual mobility may shift due to post-translational modifications; include appropriate controls.
• In immunohistochemistry (IHC-P), antigen retrieval conditions (e.g., citrate buffer pH 6.0 or Tris-EDTA pH 9.0) should be optimized as nuclear targets can require stronger retrieval.
• For ELISA, use the immunogen fragment as a positive control and determine optimal coating concentration experimentally.
• As a polyclonal antibody, it may exhibit batch-to-batch variability; include internal positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, streamlining your access to validated antibodies, antibody pairs, and specialized reagents. This SART3 polyclonal antibody targets a key spliceosome factor relevant to cancer and HIV research, and CamelBio can support your workflow with bulk supply, optimized monoclonal/polyclonal alternatives, and ancillary reagents. Let CamelBio be your partner in building robust diagnostic assays from development to clinical application.