Anti-SART1 Polyclonal Antibody (Rabbit pAb) for WB, IHC-P, IP, ELISA - O43290

Core Product Specifications and Parameters

Biological Background: SART1 Function and Localization

• SART1, also known as SNU66 homolog (hSnu66), is a U4/U6.U5 tri-snRNP-associated 110 kDa protein, originally identified as a squamous cell carcinoma antigen recognized by cytotoxic T-cells (SART-1).
• Acts as a component of the U4/U6-U5 tri-snRNP complex, a critical building block of the spliceosome, thereby playing a pivotal role in pre-mRNA splicing.
• May also bind to DNA, suggesting possible additional functions beyond splicing.
• Localizes predominantly to the nucleus, consistent with its function in mRNA processing.
• Expressed ubiquitously across tissues, indicating a fundamental cellular housekeeping role.
• Post-translational modifications include phosphorylation (phosphoprotein) and isopeptide bond formation (Ubl conjugation); undergoes alternative splicing to generate multiple isoforms.
• Classified under Keywords: 3D-structure, Allergen, Coiled coil, Isopeptide bond, mRNA processing, mRNA splicing, Nucleus, Phosphoprotein, Proteomics identification, Reference proteome, Spliceosome, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein encompassing amino acids 1–255 of human SART1 (NP_005137.1), targeting the N-terminal domain. Consider this region when designing detection strategies.
• In western blot (WB) applications, the predicted molecular weight is ~90 kDa; confirm band size in the relevant sample system.
• Reported cross-reactivity with human, mouse, and rat allows for multi-species studies; appropriate controls are recommended.
• For immunohistochemistry (IHC-P), standard antigen retrieval methods can be tried; optimization for specific tissue types may be necessary.
• This polyclonal antibody is also suitable for immunoprecipitation (IP) and ELISA; perform titration experiments to determine optimal working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials. This anti-SART1 polyclonal antibody, targeting the spliceosomal protein involved in mRNA processing and cancer immunity, is part of our comprehensive portfolio. Through CamelBio, you can source validated antibody pairs, bulk ancillary reagents, and customized rare-target raw materials to support your IVD development from concept to clinic.