Anti-SARS/Seryl-tRNA Synthetase (SARS1) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P49591

Core Product Specifications and Parameters

Biological Background: SARS (Seryl-tRNA Synthetase / SARS1) Function and Localization

• SARS (also designated serine--tRNA ligase, cytoplasmic, Seryl-tRNA synthetase, SerRS) is encoded by the SARS1 gene and belongs to the class II aminoacyl-tRNA synthetase family.
• Catalyzes the ligation of serine to its cognate tRNA(Ser) in a two-step mechanism: serine activation by ATP to form Ser-AMP, followed by transfer to the 3'-end of tRNA(Ser), essential for accurate protein translation. Related references: PMID:22353712 PMID:24095058 PMID:26433229
• Also capable of misacylating tRNA(Sec) with serine to produce L-seryl-tRNA(Sec), the precursor for selenocysteine biosynthesis, thereby contributing to selenoprotein production. Related references: PMID:26433229 PMID:28236339 PMID:34570399
• In the nucleus, directly binds the VEGFA core promoter, blocking MYC-mediated transcriptional activation, and additionally recruits SIRT2 to deacetylate histone H4K16, leading to epigenetic silencing of VEGFA and suppression of angiogenesis. Related references: PMID:24940000 PMID:19423847 PMID:19423848
• Localizes to both cytoplasm and nucleus, reflecting its dual roles in translation and transcriptional regulation.
• Predominantly expressed in brain tissue, consistent with neurological disease associations.
• Post-translational modifications include acetylation and phosphorylation; mutations are implicated in epilepsy and intellectual disability (see UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 300 amino acids of human SARS; thus the antibody may detect full-length endogenous protein and potential N-terminal-containing isoforms. Confirm specificity using appropriate controls.
• For Western blotting, expect a band at approximately 59 kDa. Optimize loading and blocking conditions to minimize nonspecific binding typical of polyclonal antibodies.
• In immunohistochemistry (IHC-P), both nuclear and cytoplasmic staining patterns may be observed; validate with known positive/negative tissues such as brain.
• In immunofluorescence/immunocytochemistry (IF/ICC), monitor subcellular localization; consider co-staining with compartment markers.
• For ELISA, titrate antibody and use validated recombinant protein as standard; cross-reactivity with mouse and rat predicted but should be verified experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, offering a comprehensive range of validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. This anti-SARS polyclonal antibody supports translational and transcriptional research, and we can assist with bulk supply of this antibody as well as customized sourcing for rare targets in aminoacyl-tRNA synthetase pathway. Our integrated services streamline procurement from discovery to clinical application.