Anti-SARS Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P49591

Core Product Specifications and Parameters

Biological Background: SARS Function and Localization

• The SARS gene encodes cytoplasmic seryl-tRNA synthetase (SerRS), also known as seryl-tRNA(Ser/Sec) synthetase, a class II aminoacyl-tRNA synthetase essential for protein translation. Alternative names include SERS, SERRS, and NEDMAS.
• Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction: serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser) (PubMed:22353712, PubMed:24095058, PubMed:26433229). Related references: PMID:22353712, PMID:24095058, PMID:26433229
• Also likely able to aminoacylate tRNA(Sec) with serine, forming misacylated L-seryl-tRNA(Sec), which is subsequently converted to selenocysteinyl-tRNA(Sec) for selenoprotein synthesis (PubMed:26433229, PubMed:28236339, PubMed:34570399). Related references: PMID:26433229, PMID:28236339, PMID:34570399
• In the nucleus, SerRS binds the VEGFA core promoter and prevents MYC binding and transcriptional activation. It recruits SIRT2 to the VEGFA promoter, promoting deacetylation of histone H4 at Lys-16 (H4K16), thereby inhibiting VEGFA production and sprouting angiogenesis (PubMed:19423847, PubMed:19423848, PubMed:24940000). Related references: PMID:19423847, PMID:19423848, PMID:24940000
• Localizes to both the cytoplasm and nucleus, consistent with its dual roles in translation and transcriptional regulation.
• Shows tissue-specific expression in the brain, and mutations in SARS are associated with neurological disorders such as epilepsy and intellectual disability.
• Post-translationally modified by acetylation and phosphorylation, and the protein structure has been determined by X-ray crystallography (3D-structure keyword).

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal region (aa 1-300) of human SARS; verify that this epitope is accessible in your experimental system, particularly for IHC-P and ELISA.
• For Western blot, the predicted molecular weight is approximately 59 kDa; use appropriate positive and negative controls from human, mouse, or rat tissues.
• This polyclonal antibody may detect multiple isoforms or degradation products; consider using blocking peptide or siRNA knockdown to confirm specificity.
• In IHC-P, optimize antigen retrieval and antibody dilution; include nuclear and cytoplasmic staining patterns as internal controls.
• Cross-reactivity with mouse and rat SARS allows for functional studies in these model organisms; however, validate reactivity in relevant tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop platform for IVD raw-material sourcing, offering bulk and customized antibodies, antigens, and ancillary reagents to support diagnostic development. This SARS (seryl-tRNA synthetase) polyclonal antibody exemplifies our capability to deliver tools for aminoacyl-tRNA synthetase research, including rare targets and transcription/translation studies. Whether you need validated antibody pairs for assay development or rare protein reagents, CamelBio streamlines your procurement from concept to clinic.