Anti-SARS-CoV Spike RBD Monoclonal Antibody for WB, ELISA - P59594

Core Product Specifications and Parameters

Biological Background: SARS-CoV Spike RBD Function and Localization

• The Spike glycoprotein, also known as E2 or peplomer protein, is a 139 kDa class I viral fusion protein encoded by the S gene of SARS-CoV (UniProt P59594).
• It mediates viral attachment to host cells by binding to receptors such as human ACE2 and CLEC4M/DC-SIGNR, triggering internalization into endosomes (By similarity).
• Following endocytosis, cathepsin CTSL proteolysis unmasks the fusion peptide in the S2 subunit, activating membrane fusion between the viral envelope and host endosomal membrane.
• The protein also down-regulates host tetherin (BST2) via lysosomal degradation, counteracting innate antiviral activity.
• Subcellular localization includes the virion membrane, host endoplasmic reticulum-Golgi intermediate compartment membrane, and host cell membrane.
• Structural features include a signal peptide, coiled coil heptad repeats, disulfide bonds, a transmembrane helix, and palmitoylation, all essential for its fusogenic function.
• Keywords: Viral envelope protein, virus entry, host-virus interaction, immunoevasion, fusion of virus membrane with host membrane.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 350–450 of the Spike RBD; the resulting antibody is expected to recognize the native RBD in Western blot under reducing or non-reducing conditions.
• For ELISA, consider this monoclonal antibody as a capture or detection reagent; matrix effects and dynamic range should be empirically determined.
• Validate specificity and cross-reactivity in the relevant experimental system (e.g., SARS-CoV infected cell lysates or recombinant RBD proteins).
• No cross-reactivity data are provided for other coronaviruses; testing is recommended if broad reactivity is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for SARS-CoV spike research and diagnostic development. We offer validated monoclonal antibodies like this anti-RBD clone, alongside optimized antibody pairs, bulk ancillary reagents, and sourcing support for rare targets. Reach out to explore how CamelBio can accelerate your project from concept to clinic.