Anti-SARS-CoV Spike Polyclonal Antibody for WB & ELISA - P59594

Core Product Specifications and Parameters

Biological Background: SARS-CoV Spike Function and Localization

• SARS-CoV Spike glycoprotein (also known as E2 or peplomer protein) is encoded by the S gene and has a molecular weight of approximately 139 kDa. It is a class I viral fusion protein essential for SARS-CoV entry.
• The protein attaches the virion to host cell receptors, primarily human ACE2 and CLEC4M/DC-SIGNR, initiating infection. Upon receptor binding, the virus is internalized into endosomes.
• Endosomal acidification triggers cathepsin L (CTSL) proteolysis, which cleaves the Spike protein and unmasks the fusion peptide in the S2 subunit, activating membrane fusion.
• Spike mediates fusion of viral and host membranes via a trimer-of-hairpins structure formed by heptad repeat regions. This conformational change drives membrane apposition and fusion.
• Additionally, Spike may down-regulate host tetherin (BST2) by targeting it for lysosomal degradation, thereby counteracting the host innate immune response.
• Subcellularly, the Spike protein is found in the virion membrane, host endoplasmic reticulum-Golgi intermediate compartment membrane, and host cell membrane.
• Keywords associated with the protein include viral envelope protein, host-virus interaction, viral immunoevasion, and viral penetration into host cytoplasm, reflecting its multifaceted role in infection.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 600–700 of the Spike protein (NP_828851.1), which lies within the S2 subunit. This antibody is expected to detect both full-length Spike and the S2 cleavage product.
• For Western blot (WB), a band near 139 kDa corresponding to full-length Spike may be observed, and under reducing conditions, S2 fragments may also appear. Consider using appropriate positive controls, such as SARS-CoV-infected cell lysates or recombinant Spike protein.
• In ELISA applications, the antibody can be used for capture or detection of SARS-CoV Spike protein. Optimize coating and blocking conditions, and validate specificity against other coronaviruses as needed.
• Since this is a polyclonal antibody, it may exhibit batch-to-batch variability. It is recommended to titrate the antibody for each application and confirm performance in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This anti-SARS-CoV Spike polyclonal antibody exemplifies our capability to supply validated antibodies for infectious disease research and assay development. Beyond this product, CamelBio offers optimized monoclonal/polyclonal antibody pairs, bulk ancillary reagents, and can assist in sourcing rare-target raw materials to accelerate your diagnostic projects.