Anti-SARS-CoV-2 Spike RBD Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P0DTC2

Core Product Specifications and Parameters

Biological Background: Spike glycoprotein Function and Localization

• The spike glycoprotein (S) is a precursor fusion protein that mediates viral entry by binding to host receptor ACE2 and triggering membrane fusion. Related references: PMID:32142651, PMID:32155444, PMID:33607086
• Upon cleavage, the S2 subunit exposes fusion peptides for direct cell membrane fusion; alternatively, uncleaved S leads to endocytosis and fusion via endosomal pathways. Related references: PMID:34561887, PMID:32075877, PMID:32221306
• Alternative host receptors include NRP1/NRP2, integrins, and ASGR1, enabling ACE2-independent entry and contributing to tissue tropism. Related references: PMID:33082294, PMID:33082293, PMID:35150743, PMID:34837059
• The S protein is cleaved by host proteases TMPRSS2 or cathepsin L (CSTL) during entry, triggering irreversible conformational changes. Related references: PMID:32703818, PMID:34159616
• Post-translational modifications include glycosylation, disulfide bonds, and palmitoylation, which are critical for structure and function.
• Subcellular localization: virion membrane, host ER-Golgi intermediate compartment membrane, and host cell membrane.
• Contains a receptor-binding domain (RBD) within the S1 subunit that directly interacts with ACE2, making it a key target for neutralizing antibodies.
• May act as a superantigen and inhibit host innate immune responses, contributing to viral immunoevasion.

Experimental Guidance and Technical Tips

• The immunogen encompasses the receptor-binding domain (aa 332-550) of the SARS-CoV-2 spike protein, making this antibody suitable for detecting the RBD in assays such as western blotting (WB), ELISA, and immunoprecipitation (IP).
• For immunofluorescence (IF/ICC), consider validating the antibody in infected cell lines or spike-transfected cells under appropriate fixation and permeabilization conditions.
• As a polyclonal antibody, it may recognize multiple epitopes on the RBD; perform titration and include proper controls (e.g., negative cell lysates, isotype controls) to ensure specificity.
• Cross-reactivity is reported for SARS-CoV-2; reactivity with other coronaviruses or variants should be experimentally confirmed.
• The predicted molecular weight of the full-length spike protein is approximately 141 kDa; however, cleaved or glycosylated forms may migrate differently; consider running a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, academic labs, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For SARS-CoV-2 research, we offer a range of spike-targeting antibodies and antigens to support viral entry studies, assay development, and vaccine evaluation. As your one-stop sourcing bridge, CamelBio simplifies procurement from pilot-scale screening to large-scale manufacturing, ensuring reliable supply for your IVD projects.