Anti-SARS-CoV-2 Spike ECD Polyclonal Antibody for WB, ELISA - P0DTC2

Core Product Specifications and Parameters

Biological Background: SARS-CoV-2 Spike Glycoprotein Function and Localization

• The spike glycoprotein (S, E2, peplomer protein) is a class I viral fusion protein that mediates SARS-CoV-2 entry into host cells by binding to the ACE2 receptor (PubMed:32142651, PubMed:32155444, PubMed:33607086). Related references: PMID:32142651 PMID:32155444 PMID:33607086
• Upon receptor binding, cleavage at the S2/S2' site by host proteases TMPRSS2 or cathepsin L (CSTL) triggers membrane fusion, either at the cell surface or within endosomes (PubMed:32142651, PubMed:32703818, PubMed:34159616). Related references: PMID:32142651 PMID:32703818 PMID:34159616
• The spike protein can also use alternative receptors such as NRP1/NRP2 and integrins, contributing to tropism for olfactory epithelial cells and ACE2-independent entry pathways (PubMed:33082293, PubMed:33082294, PubMed:35150743). Related references: PMID:33082293 PMID:33082294 PMID:35150743
• The spike ectodomain (ECD) comprises the S1 receptor-binding subunit and the S2 fusion subunit, with flexible hinges in the stalk allowing dynamic orientation (PubMed:32817270). Related references: PMID:32817270
• Subcellularly, the spike protein is found on the virion membrane, host endoplasmic reticulum-Golgi intermediate compartment membrane, and host cell membrane.
• The protein is heavily glycosylated and palmitoylated, featuring disulfide bonds and coiled-coil regions characteristic of viral fusion machinery.

Experimental Guidance and Technical Tips

• The immunogen was a recombinant fusion protein covering the entire extracellular domain (amino acids 11–1208), making the antibody suitable for detecting full-length and soluble spike ECD forms.
• For Western blot under reducing conditions, consider using a denaturing sample buffer with β-mercaptoethanol or DTT, as the spike protein contains multiple disulfide bonds.
• This antibody is validated for ELISA, potentially suitable for spike antigen capture or binding assays; optimization of coating and blocking conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop sourcing partner for IVD manufacturers and researchers, providing comprehensive access to raw materials, technical support, and consulting from concept to clinic. This anti-SARS-CoV-2 spike ECD polyclonal antibody exemplifies our expertise in supplying high-quality immunological reagents for infectious disease research. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare or emerging targets.