Anti-SARS-CoV-2 Nucleoprotein Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P0DTC9

Core Product Specifications and Parameters

Biological Background: SARS-CoV-2 Nucleoprotein Function and Localization

• Nucleoprotein (N) packages the positive-strand viral genomic RNA into a helical ribonucleocapsid, playing a fundamental role in virion assembly via interactions with viral RNA and membrane (M) protein. It also enhances subgenomic viral RNA transcription and replication, while attenuating host stress granule formation by limiting G3BP1 access to mRNAs. Related references: PMID:33264373, PMID:34901782, PMID:36534661
• Secreted N protein may block host chemokine function by competing for glycosaminoglycan binding, facilitating immune evasion and viral transmission. Related references: PMID:35921414
• N protein can induce inflammasome responses by interacting with host NLRP3, disrupting NLRP3-ABHD8 binding and enhancing NLRP3 stability, potentially contributing to cytokine release and COVID-19 lung injury. Related references: PMID:39225180
• Subcellular localization spans the virion, host cytoplasm, secreted fraction, and host extracellular space, reflecting its multifaceted roles in the viral life cycle and host interaction.
• Post-translational modifications reported include acetylation, ADP-ribosylation, phosphorylation, and ubiquitin conjugation, as indicated by UniProt keywords.
• Classified as a viral nucleoprotein with RNA-binding, ribonucleoprotein, transcription, and transcription regulation functions; plays a central role in coronavirus pathogenicity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-420 of SARS-CoV-2 nucleoprotein (P0DTC9), covering the full-length protein. This design may provide broad epitope recognition.
• Predicted molecular weight is 46 kDa; for Western blot, consider appropriate gel percentages and transfer conditions. Include positive controls (e.g., SARS-CoV-2 infected cell lysates) to confirm reactivity.
• Reported applications include IF/ICC and ELISA. For IF/ICC, fixative and permeabilization conditions should be optimized for viral antigen detection; for ELISA, pair with a compatible detection antibody and validate in relevant sample matrices.
• Cross-reactivity is reported with SARS-CoV-2; performance with other coronaviruses should be verified experimentally if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes one-stop access to validated IVD raw materials, technical services, and consulting. Our portfolio includes this SARS-CoV-2 nucleoprotein rabbit monoclonal antibody for COVID-19 research and assay development. We support customers with optimized antibody pairs, bulk ancillary reagents, and rare target sourcing—bridging the gap from concept to clinic.