Anti-SARS-CoV-2 N Protein Monoclonal Antibody for WB, IF/ICC, ELISA - P59595

Core Product Specifications and Parameters

Biological Background: SARS-CoV Nucleoprotein Function and Localization

• SARS-CoV nucleoprotein (P59595), also known as Nucleocapsid protein (NC, Protein N), is a ~46 kDa structural protein encoded by the N gene. It shares high sequence identity with SARS-CoV-2 N protein, supporting cross-reactivity of this antibody.
• The protein packages the positive-strand viral genomic RNA into a helical ribonucleocapsid (RNP) and is essential for virion assembly through interactions with the viral genome and membrane protein M.
• It enhances the efficiency of subgenomic viral RNA transcription and viral replication. Related references: PMID:17210170
• N protein may modulate host transforming growth factor-beta signaling by binding to host SMAD3, potentially influencing pathogenesis. Related references: PMID:18055455
• Subcellular localization includes the virion, host endoplasmic reticulum-Golgi intermediate compartment, host Golgi apparatus, host cytoplasm, perinuclear region, and host nucleus.
• Post-translational modifications include ADP-ribosylation and phosphorylation. The protein functions as an RNA-binding ribonucleoprotein involved in transcription regulation.
• Keywords: 3D-structure, ADP-ribosylation, Host cytoplasm, Host Golgi apparatus, Host nucleus, Host-virus interaction, Phosphoprotein, Reference proteome, Ribonucleoprotein, RNA-binding, Transcription, Transcription regulation, Viral nucleoprotein, Virion.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–420 of coronavirus Nucleoprotein (P0DTC9), encompassing nearly the full-length protein. Verify antibody specificity using recombinant N protein or SARS-CoV-2 infected cell lysates in your experimental system.
• For Western blot (WB), a predominant band near 46 kDa is expected; use appropriate loading controls and validate detection in relevant lysates.
• In immunofluorescence (IF/ICC), the antibody may label cytoplasmic, perinuclear, or nuclear compartments depending on infection stage. Employ proper fixation/permeabilization protocols and include uninfected controls.
• For ELISA, consider pairing with a capture antibody or using direct antigen coating; optimize conditions for your specific sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material partner supporting diagnostic developers from concept to clinic. For SARS-CoV-2 N protein research, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents in bulk. Whatever your assay format—ELISA, lateral flow, or molecular probes—we connect you to reliable raw materials and technical consultation tailored to your platform.