Anti-SARS-CoV-2 N Protein Monoclonal Antibody for WB, ELISA - P59595

Core Product Specifications and Parameters

Biological Background: SARS-CoV-2 N Protein Function and Localization

• The nucleoprotein (N), also known as nucleocapsid protein (NC) or protein N, is encoded by gene N and is a structural component of coronaviruses, including SARS-CoV and SARS-CoV-2, exhibiting high sequence similarity.
• N protein packages the positive-strand viral RNA genome into a helical ribonucleocapsid (RNP) and is essential for virion assembly through interactions with the viral genome and membrane protein M.
• It enhances the efficiency of subgenomic viral RNA transcription and viral replication, playing a critical role in the viral life cycle. Related references: PMID:17210170
• N protein may modulate host TGF-beta signaling by binding host SMAD3, potentially influencing host immune responses. Related references: PMID:18055455
• Subcellular localization includes the virion, host endoplasmic reticulum-Golgi intermediate compartment, host Golgi apparatus, host cytoplasm (perinuclear region), and host nucleus, reflecting its dynamic interactions during infection.
• The protein is subject to post-translational modifications such as phosphorylation and ADP-ribosylation, and is characterized by RNA-binding, transcription regulation, and ribonucleoprotein complex formation activities.

Experimental Guidance and Technical Tips

• Since the immunogen is full-length recombinant SARS-CoV-2 N protein, this antibody is expected to recognize native N protein; however, performance should be validated in the relevant sample system (e.g., infected cell lysates or purified virions).
• For WB applications, consider using a positive control such as recombinant N protein or lysates from SARS-CoV-2 infected cells; optimize antibody dilution by titration.
• In ELISA, pairing with a capture antibody may be needed; ensure proper assay conditions and validate with known positive and negative samples.
• Note that the antibody is provided in a BSA- and glycerol-free format, which may reduce interference in certain downstream applications; confirm compatibility with your detection system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibodies, bulk ancillary reagents, and custom sourcing services to support diagnostic manufacturers from concept to clinic. This anti-SARS-CoV-2 N protein monoclonal antibody is part of our expanding catalog for infectious disease research and assay development. Explore our full portfolio for rare target reagents, optimized antibody pairs, and technical support tailored to your project needs.