Anti-SARS-CoV-2 3CLpro Monoclonal Antibody for WB, IF/ICC, ELISA - P0DTC2

Core Product Specifications and Parameters

Biological Background: Spike glycoprotein Function and Localization

• The spike glycoprotein (S) is a trimeric class I fusion protein that mediates viral entry by binding to the host receptor angiotensin-converting enzyme 2 (ACE2) (PubMed:32142651, PubMed:32155444, PubMed:33607086). Related references: PMID:32142651 PMID:32155444 PMID:33607086
• Upon receptor binding, the S protein undergoes proteolytic cleavage at the S2' site by host proteases such as TMPRSS2 or cathepsin L, triggering irreversible conformational changes that drive membrane fusion (PubMed:32142651, PubMed:32703818, PubMed:34159616, PubMed:34561887). Related references: PMID:32142651 PMID:32703818 PMID:34159616 PMID:34561887
• The spike protein is also capable of using alternative receptors for entry, including neuropilin-1 (NRP1) and neuropilin-2 (NRP2), which may contribute to infection of olfactory epithelial cells with low ACE2 expression (PubMed:33082294, PubMed:33082293). Related references: PMID:33082294 PMID:33082293
• Additionally, asialoglycoprotein receptor 1 (ASGR1) can serve as an alternative receptor in an ACE2-independent manner (PubMed:34837059). Related references: PMID:34837059
• The S protein localizes to the virion membrane and is also found on the host endoplasmic reticulum–Golgi intermediate compartment (ERGIC) and host cell membrane during infection.
• It contains two heptad repeat regions (HR1 and HR2) that form a six-helix bundle structure critical for membrane fusion, and the protein is heavily glycosylated with multiple disulfide bonds and palmitoylation sites.
• The spike protein also acts as a superantigen and inhibits host innate immune responses, including tetherin antagonism, contributing to immune evasion.

Experimental Guidance and Technical Tips

• For Western blot (WB), a band at ~34 kDa (3CLpro monomer) may be observed; consider using protease inhibitors during sample preparation to prevent degradation of the viral protease.
• In immunofluorescence (IF/ICC), ensure proper fixation and permeabilization for detection of cytoplasmic 3CLpro in SARS-CoV-2-infected cells; validate in appropriate positive-control cell lines.
• ELISA applications may require titration of the antibody and recombinant 3CLpro antigen; consider a sandwich ELISA format for high sensitivity.
• Due to sequence conservation, cross-reactivity with 3CLpro from other coronaviruses cannot be excluded; verify specificity in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a streamlined pathway to source IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. As part of our portfolio for infectious disease research, this SARS-CoV-2 3CLpro antibody supports COVID-19-related assay development. Contact our team for custom sourcing of rare targets or technical consulting from concept to clinic.