Anti-SARNP Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P82979

Core Product Specifications and Parameters

Biological Background: SARNP Function and Localization

• SARNP (SAP domain-containing ribonucleoprotein), also known as CIP29, Hcc-1, THO1, and HSPC316, is a 24 kDa protein that belongs to the TREX (transcription/export) complex, essential for coupling transcription, processing, and nuclear export of spliced mRNAs.
  Related references:
  PMID:15338056
  PMID:17196963
  PMID:20844015
• It binds both single-stranded and double-stranded DNA with higher affinity for single-stranded form, specifically recognizing scaffold/matrix attachment region (S/MAR) DNA, and also binds single-stranded RNA, suggesting roles in chromatin organization and RNA metabolism.
• SARNP is a core component of the TREX complex, which is recruited in a splicing- and cap-dependent manner to mature mRNAs and facilitates their export to the cytoplasm via the TAP/NXF1 pathway; it associates with DDX39B, enhancing the latter's RNA binding and mRNA export function.
  Related references:
  PMID:37578863
• Subcellularly, SARNP localizes predominantly to the nucleus, particularly nuclear speckles, consistent with its role in pre-mRNA processing and export.
• Post-translational modifications include acetylation and phosphorylation, which likely regulate its activity and interactions.
• Expression is low in most normal tissues, but significantly up-regulated in hepatocellular carcinoma and pancreatic adenocarcinoma, implicating SARNP in cancer cell proliferation and metabolism.

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody is a recombinant protein encompassing the full-length human SARNP (amino acids 1–210). This design may provide broad epitope recognition; consider using appropriate peptide blocking controls when needed.
• For Western blot (WB), expect a band at approximately 24 kDa. Validate specificity in relevant human cell lysates; low expression in normal tissues may require using cancer cell lines or enriched nuclear extracts as positive controls.
• For immunohistochemistry (IHC), antigen retrieval conditions may be required. Since SARNP is primarily nuclear, optimal detection may depend on effective nuclear permeabilization. High expression in hepatocellular carcinoma and pancreatic adenocarcinoma suggests using these tumor tissues as positive references.
• In immunofluorescence/ICC, staining should localize to the nucleus with speckled pattern. Co-staining with nuclear markers is recommended to confirm localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a streamlined, one-stop channel for IVD raw material sourcing, bespoke technical services, and expert consultations—spanning every stage from concept to clinic. Our SARNP antibody portfolio, validated for multiple immunodetection applications, supports research into mRNA processing, cancer biology, and proliferation pathways. Beyond individual reagents, CamelBio offers comprehensive solutions including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material procurement, empowering your diagnostic and translational projects with reliable supply chains.